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目的研究T2DM伴糖尿病神经病变患者外周血单核细胞TOLL样受体4(TLR4)的表达及其与促炎因子TNF-α、IL-6的相关性。方法将研究对象分为正常组(NC,n=44),T2DM组(n=44),T2DM神经病变组(n=44)。分离和提取外周血单核细胞,流式细胞术和RT-PCR检测单核细胞表面TLR4蛋白和mRNA;ELISA检测血浆TNF-α和IL-6。结果 T2DM神经病变组HbA_1 c[(9.09±-1.62)%vs(8.36土1.10)%vs(5.30±0.89)%]、单核细胞TLR4蛋白[(42.02±9.69)%vs(31.27±6.87)%vs(11.96±5.54)%]和TLR4 mRNA[(2.98±1.06)vs(1.74±0.47)vs(1.12±0.52)]、血浆TNF-α[(8.75±3.14)vs(6.27土3.64)vs(3.19±1.17)Pg/m1]、IL-6[(3.63±1.81)vs(2.60±1.14)vs(1.54±0.58)pg/ml]水平均高于T2DM和NC组(P<0.01);T2DM神经病变组TLR4蛋白与TNF-α、IL-6呈正相关(r=0.631,P<0.0001;r=0.447,P=0.0023)。结论糖尿病神经病变患者单核细胞TLR4参与机体炎症反应,与糖尿病神经病变的发生发展密切相关。
Objective To investigate the expression of Toll-like Receptor 4 (TLR4) in peripheral blood mononuclear cells of patients with T2DM and diabetic neuropathy and its correlation with pro-inflammatory cytokines TNF-α and IL-6. Methods The subjects were divided into normal group (NC, n = 44), T2DM group (n = 44) and T2DM group (n = 44). Peripheral blood mononuclear cells were isolated and extracted. TLR4 protein and mRNA were detected by flow cytometry and RT-PCR. TNF-α and IL-6 in plasma were detected by ELISA. Results HbA 1 c [(9.09 ± 1.62)% vs (8.36 ± 1.82)% vs (5.30 ± 0.89)%], TLR4 protein [(42.02 ± 9.69)% vs (31.27 ± 6.87)% The levels of plasma TNF-α [(8.75 ± 3.14) vs (6.27 ± 3.64) vs (3.19 ± 5.54)% and TLR4 mRNA (2.98 ± 1.06 vs 1.74 ± 0.47 vs (1.63 ± 1.81) vs (1.54 ± 0.58) pg / ml] were higher than those in T2DM and NC groups (P <0.01); T2DM neuropathy Group TLR4 protein and TNF-α, IL-6 was positively correlated (r = 0.631, P <0.0001; r = 0.447, P = 0.0023). Conclusion TLR4, a monocyte involved in the pathogenesis of diabetic neuropathy, is involved in the inflammatory reaction of the body and is closely related to the occurrence and development of diabetic neuropathy.