论文部分内容阅读
目的应用图像分析仪研究妊娠期糖尿病对胎盘微绒毛结构的影响。方法取2004年1月至2005年12月间在青岛市妇女儿童医疗保健中心产科就诊妊娠期糖尿病(GDM)孕妇足月胎盘绒毛板区标本38例(GDM组)及正常妊娠孕妇足月胎盘绒毛板区标本30例(对照组),制成快速脱水、表面喷金镀膜标本,在扫描电镜下采用VIDAS型图像分析仪对胎盘微绒毛进行组织形态计量学测定。结果糖尿病孕妇的胎盘合体滋养层细胞微绒毛各例表面积、周长、长轴、短轴、等效圆直径、圆形度小于对照组,差异均有统计学意义(P<0.05);异形指数、形态因子大于对照组,差异均有统计学意义(P<0.05)。两组总的平均面积、周长、长轴、短轴、形态因子的频率分布也有部分差异(P<0.05)。结论妊娠期糖尿病引起的胎盘结构改变使母胎间气体及营养物质交换的面积大大减少,导致胎盘功能及代偿功能降低甚至失代偿,造成胎儿宫内缺氧及围生儿并发症的发生。
Objective To study the effect of gestational diabetes on placental microvilli structure with image analyzer. Methods From January 2004 to December 2005, 38 full-term placenta villi region (GDM) pregnant women with gestational diabetes mellitus (GDM) and pregnant women with full-term placental villi from the obstetrics department of Qingdao Women and Children’s Medical Center were enrolled. 30 samples of plate area (control group) were made into rapid dehydration and gold sprayed samples on the surface. The morphology of microvilli of placenta was determined by scanning electron microscope (VIDAS). Results The surface area, perimeter, major axis, minor axis, equivalent circular diameter and circularity of placental syncytiotrophoblast cells in diabetic pregnant women were all significantly lower than those in control group (P <0.05). The shape index , And the morphological factors were greater than those in the control group (all P <0.05). There was also a partial difference (P <0.05) in the frequency distribution of the total average area, perimeter, major axis, minor axis and morphological factors between the two groups. Conclusion The placental structure changes induced by gestational diabetes greatly reduce the area of gas and nutrient exchange between maternal and fetal placenta, resulting in the reduction of placental function and compensatory function or even decompensation, resulting in the occurrence of intrauterine hypoxia and perinatal complications.