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Objective:To explore larvicidal effects of anthelmintic drugs on Opisthorchis viverrini(O.viverrini) for alternative approach to interrupting its cycle for developing a field-based control program.Methods:The larvicidal activities of albendazole(A1),artesunate(Ar),praziquantel(Pzq) and miltefosine(Mf) on O.viverrini cercariae and mature metacercariae were investigated.Lethal concentrations(LC_(50) and LC_(95)) of these drugs were determined.Mature metacercariae previously exposed to various concentrations of the drugs were administered to hamsters.Worms were harvested 30 d post infection and worm recovery rates calculated.Al,Ar,Pzq and Mf produced morphological degeneration and induced shedding tails of cercariae after 24 h exposure.Results:The LC_(50) and LC_(95) of Al,Ar,Pzq and Mf on cercariae were 0.720 and 1.139,0.350 and 0.861,0.017 and 0.693,and 0.530 and 1.134 ppm,respectively.LC_(50) and LC_(95) of Ar on mature metacercariae were 303.643 and 446.237 ppm and of Mf were 289.711 and 631.781 ppm,respectively but no lethal effect in Pzq-and Al-treated groups(up to 1 ppt).No worms were found in hamsters administered Pzq-treated metacercariae.The adult worms from Al-treated metacercariae were significantly bigger in size compared to the control group(P<0.05).Fecundity and body width were greater in adults from Mf-treated metacercariae compared to the control group(P<0.05).Conclusions:The larvicidal effects of these drugs were high efficacy to O.viverrini cercariae but lesser efficacy to metacercariae.It should be further studied with the eventual aim of developing a field-based control program.
Objective: To explore larvicidal effects of anthelmintic drugs on Opisthorchis viverrini (O.viverrini) for alternative approach to interrupting its cycle for developing a field-based control program. Methods: The larvicidal activities of albendazole (A1), artesunate (Ar), praziquantel (Pzq) and miltefosine (Mf) on O.viverrini cercariae and mature metacercariae were investigated.Lethal concentrations (LC_ (50) and LC_ (95)) of these drugs were determined.Mature metacercariae previously exposed to various concentrations of the drugs were administered to hamsters.Worms were harvested 30 d post-infection and worm recovery rates calculated. Al, Ar, Pzq and Mf produced morphological degeneration and induced shedding tails of cercariae after 24 h exposure. Results: The LC_ (50) and LC_ (95) of Al, Ar, Pzq and Mf on cercariae were 0.720 and 1.139, 0.35 and 0.861, 0.017 and 0.693, and 0.530 and 1.134 ppm, respectively.LC_ (50) and LC_ (95) of Ar on mature metacercariae were 303.643 and 446.237 ppm and of Mf were 289.711 and 631.781 ppm, respectively but no lethal effect in Pzq-and Al-treated groups (up to 1 ppt). No worms were found in hamsters administered Pzq-treated metacercariae. adult adult worms from Al-treated metacercariae were significantly bigger in size than to control group (P <0.05) .Fecundity and body width were greater in adults from Mf-treated metacercariae compared to the control group (P <0.05) .Conclusions: The larvicidal effects of these drugs were high efficacy to O.viverrini cercariae but lesser efficacy to metacercariae. It should be further studied with the eventual aim of developing a field-based control program.