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目的 探讨胚胎睾丸异位移植到成年大鼠体内是否能生长发育 ,并具内分泌功能。方法 将胚胎睾丸分别移植至成年雄性Wistar大鼠颈部皮下和肾被膜下 (移植实验组 ) ,并设实验对照组 (摘除睾丸 )和正常对照组 ,移植实验组于移植后第 5d和 10d、2 5～ 35d和 35d以上取出移植物 ,同时断头取血 ,经离心取上清液。常规染色观察移植物的生长发育情况 ;细胞化学染色、免疫组织化学染色和放射免疫法检测移植物功能状态。结果 移植的胚胎睾丸在成年大鼠体内可生长发育至成年状 ;睾丸间质 3 β羟甾脱氢酶和油红O细胞化学染色呈阳性反应 ,睾丸间质细胞雄性激素及其受体、孕酮及其受体呈不同程度的阳性反应 ;血清睾酮、孕酮、卵泡刺激素和黄体生成激素接近正常值。结论 胚胎睾丸异位移植到成年大鼠体内后可正常发育 ,并具内分泌功能
Objective To investigate whether embryo testes heterotopic transplantation into adult rats can grow and develop, and have endocrine function. Methods Embryonic testes were transplanted into the subcutaneous and subrenal necks of the neck of adult male Wistar rats respectively. The experimental control group (testis) and normal control group were established. At the 5th and 10th day after transplantation, 2 5 ~ 35d and 35d above removed the graft, while decapitated blood, the supernatant by centrifugation. The growth and development of the grafts were observed by routine staining. The functional status of the grafts was detected by cytochemical staining, immunohistochemical staining and radioimmunoassay. Results Transplanted embryos testes in adult rats can grow to mature; testis interstitial 3 β-hydroxy steroid dehydrogenase and oil red O cells chemical staining was positive, testicular stromal cells androgen and its receptor, pregnancy Ketamine and its receptor showed varying degrees of positive reaction; serum testosterone, progesterone, follicle stimulating hormone and luteinizing hormone close to normal. Conclusion Ectopic transplantation of embryonic testes to adult rats can develop normally with endocrine function