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目的:寻找一种新型的转染效率高,毒性低的非病毒基因载体。方法:通过化学方法合成Polyimine-MPEI,然后以不同质量比包裹绿色荧光蛋白质粒,检测在COS-7细胞中的转染效率和毒性。结果:在比例从5到100之间,转染效率均比较理想,能达到1.00E+07以上,Polyimine-MPEI的毒性也很小,细胞的生长率均在80%以上,明显高于PEI25KDa对照组。结论:Polyimine-MPEI是一个很有研究前景的聚合物载体,具有高转染效率低毒性的特点,可以通过延长反应时间,增加分子量,增大转染能力。
Objective: To find a new non-viral gene vector with high transfection efficiency and low toxicity. Methods: Polyimine-MPEI was synthesized by chemical method. Then green fluorescent protein (EGFP) plasmids were coated with different mass ratios to detect transfection efficiency and toxicity in COS-7 cells. Results: The transfection efficiency was all above 5% to 100%, and reached to above 1.00E + 07. The toxicity of Polyimine-MPEI was also very low. The cell growth rate was above 80%, which was significantly higher than PEI25KDa control group. Conclusion: Polyimine-MPEI is a promising polymer carrier with high transfection efficiency and low toxicity. It can prolong the reaction time, increase the molecular weight and increase the transfection ability.