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以紫花苜蓿幼苗(Medicago sativa L.)为材料,对紫花苜蓿幼苗分别进行了2、4、6、8、10、12 h UV-B辐射处理,以白光(W)为对照,研究UV-B对其营养品质、药用价值及抗氧化性的影响。结果表明:与对照组相比,UV-B照射4、6、8、10 h的可溶性糖含量分别增加了68.4%、57.3%、71.1%、43.9%,差异均达到极显著水平(P<0.01);照射4 h后可溶性蛋白含量增加了22.4%,差异达到极显著水平(P<0.01);UV-B照射后,苯丙氨酸解氨酶(PAL)的活性于(4~10)h之间分别增加了49.2%、49.4%、66.7%、69.5%,差异均达到极显著水平(P<0.01);总酚、类黄酮、花青素等紫外吸收物质的含量显著提高:照射4 h时,总酚含量增加了5.3%,差异达到极显著水平(P<0.01);照射10 h时,类黄酮含量增加了4.6%,差异达到极显著水平(P<0.01);花青素含量于照射8、10、12 h时分别增加了44.4%、81.8%、63.7%,差异均达到极显著水平(P<0.01);此外,UV-B辐射后的紫花苜蓿幼苗抗氧化能力明显提高,照射12 h时增加了7.8%,差异达到极显著水平(P<0.01)。由此可见,UV-B辐射可以显著提高紫花苜蓿幼苗的品质及抗氧化能力。
The alfalfa seedlings were treated with UV-B radiation for 2, 4, 6, 8, 10 and 12 h, respectively. The white light (W) was used as the control to study the effects of UV-B Its nutritional quality, medicinal value and antioxidant effects. The results showed that the soluble sugar content increased by 68.4%, 57.3%, 71.1% and 43.9% at 4, 6, 8 and 10 h after UV-B irradiation compared with the control group, all of which reached the extremely significant level ). The content of soluble protein increased 22.4% 4 h after irradiation, and the difference reached a significant level (P <0.01). After UV-B irradiation, the activity of PAL was decreased from (4 ~ 10) h (49.2%, 49.4%, 66.7% and 69.5% respectively). The content of UV absorbers, such as total phenols, flavonoids and anthocyanin, increased significantly at 4 h (P <0.01). The content of flavonoids increased by 4.6% at 10 h after irradiation (P <0.01), and the content of anthocyanin at the level of extremely significant Compared with the control group, the anti-oxidation ability of alfalfa seedlings after UV-B radiation increased significantly by 44.4%, 81.8% and 63.7% at 8, 10 and 12 hours, respectively. The difference was significant (P <0.01) Increased by 7.8% at 12 h, the difference reached extremely significant level (P <0.01). Thus, UV-B radiation can significantly improve the quality of alfalfa seedlings and antioxidant capacity.