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目的对原发性肝癌患者外周血NK细胞数目、亚群及其杀伤功能进行检测,分析原发性肝癌外周血中NK细胞的特点及机制。方法临床确诊原发性肝癌(HCC)患者110例,健康对照69例,采用流式细胞仪检测外周血NK细胞的数目及亚群;将IL-2活化的PBMC与CFSE标记的K562细胞混合,流式细胞仪检测NK细胞的杀伤活性。结果 1)Ⅰ、Ⅱ、Ⅲ期原发性肝癌患者外周血NK细胞占淋巴细胞的比率均显著低于健康对照(P均<0.01);2)与健康对照相比,Ⅰ、Ⅱ期原发性肝癌患者外周血CD56bright NK细胞占淋巴细胞的比率无显著变化(P>0.05),Ⅲ期原发性肝癌患者CD56bright NK细胞占淋巴细胞的比率显著升高(P<0.05);3)Ⅰ、Ⅱ、Ⅲ期原发性肝癌患者外周血CD56dimNK细胞占淋巴细胞的比率显著低于健康对照(P均<0.01);4)Ⅰ、Ⅱ、Ⅲ期原发性肝癌患者外周血NK细胞CD56bright/CD56dim NK cells值显著高于健康对照(P均<0.01);5)原发性肝癌患者外周血NK细胞裂解杀伤靶细胞K562细胞的活性与健康对照相比显著降低(P<0.05)。结论原发性肝癌患者外周血NK细胞数量显著降低,可能是CD56bright NK细胞的显著增高和CD56dimNK细胞显著降低综合作用的结果。原发性肝癌患者外周血NK细胞的杀伤活性显著降低,可能与CD16+NK细胞减少有关。提示以NK为代表的天然免疫系统参与了肝癌的发生与发展。
Objective To detect the number of NK cells in peripheral blood of patients with primary liver cancer, its subgroup and its killing function, and to analyze the characteristics and mechanism of NK cells in peripheral blood of patients with primary liver cancer. Methods 110 cases of primary hepatocellular carcinoma (HCC) and 69 healthy controls were detected clinically. The numbers and subpopulations of peripheral blood NK cells were detected by flow cytometry. IL-2-activated PBMCs were mixed with CFSE-labeled K562 cells, Cytotoxicity detection of NK cells by flow cytometry. Results 1) The ratios of NK cells to lymphocytes in peripheral blood of patients with primary liver cancer in stages Ⅰ, Ⅱ and Ⅲ were significantly lower than those in healthy controls (all P <0.01); 2) Compared with healthy controls, (P> 0.05). The ratio of CD56bright NK cells to lymphocytes in patients with stage Ⅲ primary hepatocellular carcinoma was significantly increased (P <0.05). 3) The expression of CD56bright NK cells in peripheral blood of patients with primary hepatocellular carcinoma The ratio of CD56dimNK cells to lymphocytes in peripheral blood of patients with primary liver cancer in stage Ⅱ and Ⅲ was significantly lower than that in healthy controls (all P <0.01); 4) The levels of CD56bright / CD56dim (P <0.01). 5) The activity of K562 cells in K562 cells was significantly lower than that of healthy controls in patients with primary liver cancer (P <0.05). Conclusion The number of NK cells in peripheral blood of patients with primary liver cancer is significantly lower than that of patients with primary liver cancer, which may be the result of the significant increase of CD56bright NK cells and the significant reduction of CD56dimNK cells. The cytotoxic activity of NK cells in peripheral blood of patients with primary liver cancer is significantly reduced, which may be related to the decrease of CD16 + NK cells. Prompted to NK as the representative of the natural immune system involved in the occurrence and development of liver cancer.