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目的检测血小板活化依赖性颗粒表面膜蛋白(CD62P)在糖尿病肾病患者血浆中的表达水平,探讨它在糖尿病肾病发病机制中的作用。方法58例糖尿病人根据尿蛋白排泄率(UAE)及肾功能变化分为三组,正常尿蛋白组;尿蛋白持续阳性肾功能正常组;肾功能异常组。并设立对照组。流式细胞仪检测CD62P。同时检测血糖,肾功能,行血小板及血小板分布宽度检测;行即日尿白蛋白排泄率(UAE)检验;记录患者的血压。结果血CD62P浓度检测:Ⅰ组与Ⅳ组比较没有显著性差异(P=0.303)、与Ⅱ组比较有显著性差异(P=0.033)、与Ⅲ组比较有显著性差异,P<0.001;Ⅱ组与Ⅳ比较有显著性差异,P<0.05,与Ⅲ组比较有显著性差异,P=0.001。血常规检测血小板及血小板分布宽度,各组之间比较没有显著性差异,P>0.05。结论CD62P在糖尿病肾病各期都有不同程度的表达,且随着糖尿病肾病病情的发展,CD62P表达更加明显。提示CD62P在糖尿病肾病的发生发展中可能起重要的作用。粘附分子CD62P的高表达与与高血糖等因素有关。CD62P介导淋巴细胞、单核细胞和T淋巴细胞聚集于肾小球和肾间质中,可能是其在DN发病中起作用的机制。在糖尿病肾病的发生发展中,CD62P的高表达不依赖于血小板数量及血小板分布宽度变化。
Objective To detect the expression of platelet-activating-dependent granule surface membrane protein (CD62P) in the plasma of patients with diabetic nephropathy and to explore its role in the pathogenesis of diabetic nephropathy. Methods 58 cases of diabetic patients were divided into three groups based on urinary protein excretion rate (UAE) and renal function changes, normal urinary protein group; urinary protein persistent normal renal function group; renal dysfunction group. And set up a control group. Flow cytometry detection of CD62P. At the same time, blood glucose, renal function, platelet and platelet distribution width were measured. The urinary albumin excretion rate (UAE) test was performed immediately. The patient’s blood pressure was recorded. Results There was no significant difference in serum CD62P between group Ⅰ and group Ⅳ (P = 0.303), there was significant difference between group Ⅰ and group Ⅱ (P = 0.033), there was significant difference between group Ⅰ and group Ⅲ There was a significant difference between group Ⅳ and group Ⅳ, P <0.05, there was a significant difference compared with group Ⅲ, P = 0.001. Blood test platelet and platelet distribution width, no significant difference between the groups, P> 0.05. Conclusion The expression of CD62P is different in all stages of diabetic nephropathy. With the development of diabetic nephropathy, the expression of CD62P is more obvious. These results suggest that CD62P may play an important role in the development of diabetic nephropathy. High expression of adhesion molecule CD62P and hyperglycemia and other factors. CD62P-mediated accumulation of lymphocytes, monocytes and T lymphocytes in the glomerulus and renal interstitium may play a role in the pathogenesis of DN. In the development of diabetic nephropathy, the high expression of CD62P does not depend on the number of platelets and platelet distribution width changes.