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目的:探讨CD44v17对宫颈癌的临床诊断意义。方法:将CD44v17si RNA、CD44v17、生理盐水转染至传代后的人宫颈癌细胞。检测细胞转染后存活率;检测细胞凋亡率。在裸鼠左肩背部注入人宫颈癌细胞悬液,随机分为CD44v17组、CD44v17si RNA组、对照组。在CD44v17组、CD44v17si RNA组裸鼠瘤体内分别注入CD44v17病毒颗粒、CD44v17si RNA病毒颗粒。检测瘤体的质量与体积。选取疑有宫颈病变患者阴道镜下活检组织80例,正常宫颈组织15例、宫颈上皮内瘤变(CIN)I级组织l5例、CIN II级15例、CIN III级组织15例和宫颈癌组织20例。检测CD44v17在不同组织中的表达量。结果:CD44v17si RNA转染的宫颈癌细胞凋亡率(19.20±2.14%)高于CD44v17转染的宫颈癌细胞凋亡率(6.13±1.08%)(P<0.05)。CD44v17组裸鼠瘤体质量(15.9±3.4)g高于对照组裸鼠瘤体质量(11.8±2.7)g(P<0.05)。CD44v17在不同组织中的表达量,按正常宫颈、CINⅠ级、CINⅡ级、CINⅢ级、宫颈癌发展过程呈递增趋势(P<0.05)。结论:CD44v17能抑制宫颈癌细胞凋亡,促进宫颈癌细胞的生长、增殖。通过降低CD44v17表达量可能是遏制CIN向宫颈癌发展的一个手段。
Objective: To investigate the clinical significance of CD44v17 in cervical cancer. Methods: CD44v17siRNA, CD44v17 and normal saline were transfected into the passaged human cervical cancer cells. Cell viability was detected after transfection; apoptosis rate was detected. Human cervical cancer cell suspension was injected into the left shoulder of nude mice and randomly divided into CD44v17 group, CD44v17si RNA group and control group. CD44v17 group, CD44v17si RNA group were injected with CD44v17 virus particles, CD44v17si RNA virus particles. Detection of tumor mass and volume. Eighty cases of colposcopic biopsies, 15 cases of normal cervical tissue, 15 cases of cervical intraepithelial neoplasia (CIN) I grade, 15 cases of CIN II grade, 15 cases of CIN III grade and 15 cases of cervical carcinoma 20 cases. The expression of CD44v17 in different tissues was detected. Results: The apoptotic rates of cervical cancer cells transfected with CD44v17siRNA (19.20 ± 2.14%) were higher than those of CD44v17 transfected cervical cancer cells (6.13 ± 1.08%) (P <0.05). The tumor mass of CD44v17 group (15.9 ± 3.4) g was higher than that of the control group (11.8 ± 2.7) g (P <0.05). The expression of CD44v17 in different tissues showed an increasing tendency (P <0.05) according to the development of normal cervix, CINⅠ, CINⅡ, CINⅢ, and cervical cancer. Conclusion: CD44v17 can inhibit the apoptosis of cervical cancer cells and promote the growth and proliferation of cervical cancer cells. By reducing the expression of CD44v17 may be a means to curb the development of CIN to cervical cancer.