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为探讨重组人钠碘同向转运体(hNIS)基因的腺病毒感染激素非依赖性前列腺癌细胞介导放射性碘治疗的可能性,通过构建巨细胞病毒(CMV)启动的重组hNIS的腺病毒(Ad-CMV-NIS),感染激素非依赖型前列腺癌PC-3细胞,进行细胞体外和荷瘤裸鼠瘤体内分析,发现感染Ad-CMV-NIS的PC-3细胞能迅速摄取125I,其摄取能力约为空病毒转染组的120倍,摄碘功能可被NaClO4特异性抑制,125I在细胞内的半排期T1/2 efflux为26.7 min,131I可特异性杀死93.0%±3.8%的PC-3细胞。感染Ad-CMV-NIS的PC-3荷瘤裸鼠,肿瘤组织摄取125I 2 h达最高((16.30±8.72)%ID/g),125I在瘤体内的有效半衰期T1/2 effective为5.4 h,131I显像肿瘤显示清晰。结果表明,Ad-CMV-NIS感染可使PC-3细胞获得摄碘功能并被131I特异性杀死,肿瘤摄取高,为进一步体内治疗提供了有力的基础。
To investigate the possibility of adenovirus-dependent hormone-independent prostate cancer cells mediated by recombinant human sodium iodide symporter (hNIS) gene mediated by radioactive iodine treatment, the recombinant hNIS adenovirus (CMV) Ad-CMV-NIS) was detected in PC-3 cells infected with hormone-independent prostate cancer cells in vitro and in vivo. The results showed that uptake of Ad-CMV-NIS into PC-3 cells could rapidly uptake 125I The ability of uptake was about 120 times higher than that of empty virus transfection group. The function of uptake of iodine was specifically inhibited by NaClO4. The half-period T1 / 2 efflux of 125I in cells was 26.7 min. The 131I specifically killed 93.0% ± 3.8% PC-3 cells. In PC-3 bearing nude mice infected with Ad-CMV-NIS, the uptake of 125I 2 was the highest (16.30 ± 8.72% ID / g) in tumor tissues, the effective half-life of 125I in tumor was T1 / 2 effective of 5.4 h, 131I imaging tumor showed clear. The results showed that Ad-CMV-NIS infection could cause PC-3 cells to get iodine-uptake function and be specifically killed by 131I, resulting in high tumor uptake, which provided a strong foundation for further in vivo treatment.