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水稻长芒是从野生稻保留下来的性状,通过人工选择培育的现代品种一般都是无芒类型。到目前为止,还没有关于控制芒的基因精细定位和克隆的报道。本实验利用国家种质资源库中有芒的亲本SLG与无芒亲本日本晴构建回交近等基因系(NILs)群体,从BC4F2回交分离群体中,选择出有芒和无芒呈3∶1分离的群体,从中选择杂合BC4F2单株构建BC4F3定位大群体。利用分离群体分组混合分析法(BSA法),筛选均匀分布水稻染色体组上1 512对SSR标记,将芒基因定位在第3染色体RM6283和RM5685之间,命名为AWN3-1。通过设计和筛选多态性标记,进一步将AWN3-1定位在Y5和Y9标记之间,遗传距离分别为0.5和0.4 cM。这些结果为克隆AWN3-1奠定了基础。
Rice Changmang is a property preserved from wild rice, and the modern varieties cultivated through artificial selection are generally non-type. To date, there are no reports of fine mapping and cloning of genes controlling Cannon. In this study, NILs were constructed from the SLGs of A. mangium and A. nipponense in the National Germplasm Bank of China. The populations of NILs from the BC4F2 backcross population were selected to be 3: 1 Isolated populations from which BC4F2 heterozygous individuals were selected to construct large populations of BC4F3 loci. A total of 1 512 pairs of SSR markers on rice chromosomes were screened by BSA method. The genes were named as AWN3-1 between RM6283 and RM5685 on chromosome 3. By designing and screening polymorphic markers, AWN3-1 was further mapped between Y5 and Y9 markers with genetic distances of 0.5 and 0.4 cM, respectively. These results laid the foundation for the cloning of AWN3-1.