论文部分内容阅读
目的:探讨黄芪注射液对大鼠睾丸扭转/复位模型的保护作用。方法:将30只健康雄性Wistar大鼠分为3组。分别为假手术对照组(A组,n=10);睾丸扭转/复位组(B组,n=10);睾丸扭转/复位+腹腔内注射黄芪注射液组(C组,n=10)。按Turner法建立睾丸扭转模型,喂养至术后7d处死,切取扭转侧睾丸检测凋亡指数(AI)及谷胱甘肽过氧化物酶活力及丙二醛含量。结果:A、B、C三组扭转侧睾丸AI分别为5.82±1.21、36.18±8.40、20.39±3.57,B、C组明显高于对照组(P(0.05),B组明显高于C组(P(0.05)。A、B、C三组扭转侧睾丸谷胱甘肽过氧化物酶活力分别为48.03±2.01、30.93±1.25、38.44±1.06U/mg;丙二醛含量分别为1.43±0.17、3.98±0.36、2.57±0.53nmol/ml,三组之间比较均有显著性差异(P(0.05)。结论:黄芪注射液可明显减少扭转侧睾丸生殖细胞凋亡,保护谷胱甘肽过氧化物酶活力,减轻脂质过氧化程度。
Objective: To investigate the protective effect of Huangqi Injection on rat testicular torsion/reduction model. Methods: Thirty healthy male Wistar rats were divided into 3 groups. They were sham-operated control group (group A, n=10); testicular torsion/reset group (group B, n=10); testicular torsion/reset plus intraperitoneal injection of astragalus injection group (group C, n=10). The testicular torsion model was established according to the Turner method and fed to the 7th day after the operation. The torsion side testis was used to measure the apoptosis index (AI), glutathione peroxidase activity and malondialdehyde content. Results: The AI of the torsion testis in groups A, B, and C were 5.82±1.21, 36.18±8.40, and 20.39±3.57, respectively. The B and C groups were significantly higher than the control group (P<0.05). The B group was significantly higher than the C group ( P(0.05).The glutathione peroxidase activities of the torsion lateralis testes in groups A, B, and C were 48.03±2.01, 30.93±1.25, and 38.44±1.06 U/mg, respectively; the malondialdehyde contents were 1.43±0.17, respectively. There were significant differences between the three groups (3.98±0.36, 2.57±0.53 nmol/ml, P(0.05). Conclusion: Huangqi injection can significantly reduce the apoptosis of germ cells in the torsion testis and protect glutathione Oxidase activity, reducing lipid peroxidation.