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对术前未接受任何抗肿瘤治疗的４７例胃及大肠癌组织标本利用反转录—聚合酶链反应（ＲＴ－ＰＣＲ）技术检测肿瘤细胞的多药耐药ｍＲＮＡ基因（ＭＤＲ１ｍＲＮＡ）表达水平，对其影响肿瘤细胞ｍｄｒ－１基因表达的因素采用多因素电子计算机Ｌｏｇｉｓｔｉｃ回归模型法进行分析。结果显示：胃及大肠癌细胞ＭＤＲ１ｍＲＮＡ表达与病人年龄、性别及肿瘤的大小、分化程度及淋巴结转移无关；与肿瘤的生长部位、分期及局部组织浸润之间差异有显著性意义。故在临床胃及大肠癌组织标本中进行ＭＤＲ１ｍＲＮＡ表达的检测，可指导化疗方案的设计，调整抗肿瘤药物的种类，达到最佳化疗目的。尤其对术中肿瘤局部浸润较重和（或）术后病理检查提示分期较差的大肠癌病人应尤为重要。
The expression of MDR1 mRNA in tumor cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) in 47 specimens of gastric and colorectal cancer tissues that did not receive any antitumor therapy before surgery. The factors affecting mdr-1 gene expression in tumor cells were analyzed by multi-factor computer logistic regression model. The results showed that the expression of MDR1 mRNA in gastric and colorectal cancer cells was not related to the patient’s age, gender, tumor size, differentiation, and lymph node metastasis. There was a significant difference between the expression of MDR1 mRNA in gastric and colorectal cancer cells and the site of tumor growth, stage, and local tissue infiltration. Therefore, the detection of MDR1 mRNA expression in clinical gastric and colorectal cancer tissue samples can guide the design of chemotherapy regimens, adjust the types of anti-tumor drugs, and achieve the best chemotherapy goals. In particular, patients with colorectal cancer who have heavier intraoperative tumor infiltration and/or postoperative pathological examinations with poor staging should be particularly important.