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目的 研究狼疮性肾炎患者外周血 T细胞交联素 V和 Fas配体 (Fas L )的表达。 方法 应用流式细胞术定量检测了 2 0名狼疮性肾炎患者和 10名正常对照者的外周血 T细胞体外培养 48h前后 Annexin V和 Fas L的表达。 结果 狼疮肾组外周血淋巴细胞计数与正常对照组相比 ,差异不显著 (P>0 .0 5 ) ;狼疮肾组 Annexin V和 Fas L表达显著高于正常对照组(P<0 .0 5 ) ,两组 Annexin V、Fas L和血沉三者间呈显著正相关 (P<0 .0 5 )。 结论 狼疮性肾炎患者的 T细胞凋亡增加 ,且与狼疮活动性呈正比。 T细胞凋亡的重要途径之一是通过 Fas L系统发生的。
Objective To investigate the expression of T-cell cross-linking factor V and Fas ligand (L-Fas ligand) in peripheral blood of patients with lupus nephritis. Methods Flow cytometry was used to detect the expression of Annexin V and Fas L in peripheral blood mononuclear cells from 20 lupus nephritis patients and 10 normal controls before and after 48 hours of in vitro culture. Results There was no significant difference in peripheral blood lymphocyte counts between lupus nephropathy group and normal control group (P> 0.05). The expressions of Annexin V and Fas L in renal lupus nephritis group were significantly higher than those in normal control group (P <0.05) ), There was a significant positive correlation between the two groups of Annexin V, Fas L and erythrocyte sedimentation rate (P <0.05). Conclusion The apoptosis of T cells in patients with lupus nephritis is increased, and it is directly proportional to the activity of lupus. One of the important pathways for T-cell apoptosis is through the Fas L system.