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目前,测定铍方法很多,荧光光度法测定铍报道也不少,其常用试剂有桑色素等。近几年来β-环糊精(β-CD)在分析化学领域中应用逐年扩大,人们利用它环上众多羟基具有亲水特性,而其内腔则疏水性的,可包合不同化合物,而使整个体系的性质有所改变。如1,4-二羟基蒽醌与β-CD形成包合物测定钪即此类应用实例,但测定铍未见报道。本文首先利用1,4-二羟基蒽醌与β-CD形成包合物,其与铍再形成新的包合物。试验研究了该包合物最佳形成条件,在1×10~(-3)mol·L~(-1)NaOH介质中,采用530nm作为激发波长,600nm为发射波长进行荧光强度测定时,其值为最大。铍在2～10ng·ml~(-1)范围内与荧光强度呈线性关系,检出限为0.3ng·ml~(-1),精密度为1.29%。包合物形成速度快,5min后荧光强度即达最大,且至少可稳定3h,试验了30多种共存物质对测定铍的影响,结果表明,方法选择性好、灵敏度高,应用到岩石矿物中痕量铍测定,结果满意。
At present, many methods for the determination of beryllium, beryllium reported by fluorescence spectrometry is also a lot less, its commonly used reagents have morin and so on. In recent years, the application of β-cyclodextrin (β-CD) in the field of analytical chemistry has been expanding year by year. People use many hydroxyl groups in its ring to have hydrophilic character, while its internal cavity is hydrophobic and can enclose different compounds. The whole system has changed its nature. Such as 1,4-dihydroxyanthraquinone and β-CD to form inclusions scandium such application examples, but the determination of beryllium has not been reported. In this paper, firstly, 1,4-dihydroxyanthraquinone and β-CD were used to form inclusion complexes, which formed new inclusion complexes with beryllium. The optimal conditions for the inclusion complex were studied experimentally. When the fluorescence intensity was measured at 530 nm as the excitation wavelength and 600 nm as the emission wavelength in 1 × 10 -3 mol·L -1 NaOH medium, The value is maximum. The beryllium showed a linear relationship with the fluorescence intensity in the range of 2 ~ 10 ng · ml ~ (-1) with a detection limit of 0.3 ng · ml -1 and a precision of 1.29%. The results show that the method has the advantages of good selectivity and high sensitivity, and is applied to rock and minerals. The results show that the inclusion complex formation speed is fast, and the fluorescence intensity reaches the maximum after 5 min, and can be stable for at least 3 h. Trace beryllium determination, the results are satisfactory.