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目的评价基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization-time of flight mass spectrometry,MALDI-TOF MS)对肺炎克雷伯菌(Klebsiella pneumoniae,KPN)同源性分析的能力。方法对2013年2-4月青岛大学附属医院重症监护病房(ICU)分离的7株KPN和其他科室分离的13株KPN进行溯源性回顾分析,采用方法为脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)、多位点序列分型(multilocus sequence typing,MLST)和MALDI-TOF MS。结果 PFGE和MLST结果一致,MALDI-TOF MS同源性分析中将20株菌株分为2类,Ⅰ类为来自ICU的7株菌,Ⅱ类为其他科室的13株菌,与前2种方法得到的结果基本一致。结论 MALDI-TOF MS技术能够准确鉴定肺炎克雷伯菌且可对其进行同源性分析,较其他同源性分析方法快捷、方便,可满足临床对院感工作的需求。
Objective To evaluate the ability of matrix-assisted laser desorption / ionization-time of flight mass spectrometry (MALDI-TOF MS) to analyze the homology of Klebsiella pneumoniae (KPN). Methods Seven KPNs isolated from ICU of Qingdao University Affiliated Hospital of Qingdao University from January to April 2013 were retrospectively analyzed retrospectively. Pulsed-field gel electrophoresis, PFGE), multilocus sequence typing (MLST) and MALDI-TOF MS. Results The results of PFGE and MLST were consistent. In the analysis of MALDI-TOF MS homology, 20 strains were divided into two groups, the first group consisted of 7 strains from ICU and the second group consisted of 13 strains from other departments. The results obtained are basically the same. Conclusion MALDI-TOF MS can accurately identify Klebsiella pneumoniae and analyze its homology. Compared with other homology analysis methods, MALDI-TOF MS is fast and convenient, and can meet the needs of clinical work on hospitalization.