论文部分内容阅读
目的 探讨细胞周期调控因子在非小细胞肺癌组织中的表达及作用。方法 应用LSAB免疫组化的方法对 12 3例原发性非小细胞肺癌进行检测。结果 5 1.2 %的肺癌出现cyclinD1的过表达 ,77.2 %的肿瘤组织表达CDK 4,并与肿瘤的分化程度呈反比。有淋巴结转移的肺癌 ,其cyclinD 1和CDK 4的阳性率分别为 87.3 %和 68.4%。无淋巴结转移的肺癌阳性率分别为12 .6%和 3 1.5 %。两者相比差异有显著性 (P <0 .0 5 )。结论 cyclinD1、CDK 4在非小细胞肺癌中过度表达 ,并与肿瘤的分化程度 ,淋巴结转移密切相关。p16的表达可能受Rb蛋白的负调控。肺癌的分子发病机制涉及cyclinD 1和CDK 4、Rb和 p16负反馈调节通路的异常。
Objective To investigate the expression and role of cell cycle regulators in non-small cell lung cancer (NSCLC). Methods 127 cases of primary non-small cell lung cancer were detected by LSAB immunohistochemistry. Results 51.2% of lung cancers showed overexpression of cyclinD1, and 77.2% of tumor tissues expressed CDK4, which was inversely proportional to the degree of tumor differentiation. Lung cancer with lymph node metastasis had a positive rate of cyclinD 1 and CDK 4 of 87.3% and 68.4%, respectively. The positive rates of lung cancer without lymph node metastasis were 12.6% and 31.5%, respectively. The difference between the two was significant (P < 0.05). Conclusion Cyclin D1 and CDK 4 are overexpressed in non-small cell lung cancer and are closely related to the degree of tumor differentiation and lymph node metastasis. The expression of p16 may be negatively regulated by Rb protein. The molecular pathogenesis of lung cancer involves abnormalities in the negative feedback regulatory pathways of cyclinD 1 and CDK 4, Rb, and p16.