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目的采用PCR-DGGE法探讨纳米山药多糖靶向制剂对肠道微生态失调模型大鼠的调节作用。方法以i.g盐酸林可霉素造成大鼠结肠炎模型,将大鼠随机分成常规治疗组,靶向制剂组和自然恢复组,分别给予相应药物治疗。灌胃10d后处死所有大鼠,采集盲肠内容物,提取总DNA,PCR-DGGE方法检测样本菌群DNA。将所有数据收集得到DGGE凝胶,采用Quantity One软件进行相似性和多样性分析,观察纳米山药多糖靶向制剂对肠道微生态失调大鼠的治疗效果。结果样本提取总DNA浓度值(ng/μL)分别为:靶向制剂组(0.180±0.005)和常规治疗组(0.175±0.006)高于自然恢复组(0.072±0.001)(P<0.05),与正常水平(0.182±0.006)相近;DGGE的多样性指数分析结果为:靶向组(23.95±2.36)和常规治疗组(24.00±1.68)的条带丰富度大于自然恢复组(19.57±2.52),差异有统计学意义(P<0.05)且接近正常对照组(25.87±2.10)。结论纳米山药多糖靶向制剂组对肠道微生态失调大鼠具有显著的调整作用,是理想的中药微生态调节剂。
OBJECTIVE: To investigate the regulatory effect of Nano-yam polysaccharide targeting agents on gut dysbiosis in rats by PCR-DGGE. Methods The model of colitis was induced by lincomycin hydrochloride in rats. The rats were randomly divided into routine treatment group, targeted preparation group and spontaneous recovery group. The rats were given the corresponding drug treatment. All rats were sacrificed 10 days after gavage, the contents of cecum were collected and the total DNA was extracted. PCR-DGGE was used to detect the DNA of the samples. DGGE gel was collected from all the data, and Quantity One software was used to analyze the similarity and diversity. The therapeutic effect of nano-yam polysaccharide targeting agent on gut microbiological disorders was observed. Results The total DNA concentration (ng / μL) in the samples was (0.180 ± 0.005) and 0.175 ± 0.006 (0.072 ± 0.001) compared with the natural recovery group (P <0.05) (0.182 ± 0.006). The results of DGGE showed that the bands richness of the target group (23.95 ± 2.36) and the routine treatment group (24.00 ± 1.68) was higher than that of the natural recovery group (19.57 ± 2.52) The difference was statistically significant (P <0.05) and close to the normal control group (25.87 ± 2.10). Conclusion Nano-yam polysaccharide targeting preparation has a significant adjustment effect on gut microecological disorders in rats, and is an ideal regulator of traditional Chinese medicine.