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维生素D 依赖性钙结合蛋白(Calbindin～D,Cal-D)是细胞一大类钙结合蛋白中的一种。以分子量区分,有28KDa 和10KDa 两型。作者用酶法测定了人类各组织和体液内28KDa Cal-D 的含量。方法:人28KDa Cal-D 的提钝:正常人肾组织匀浆经冷却、反复离心后,取经热沉淀的上清液用0.02mol/L pH7.4Tris-HCl 缓冲液(含1mmol/L EDTA,0.07mol/L NaCl,0.01%2-巯基乙醇)4℃透析过液。然后加到用上述缓冲液平衡过的DEAE-葡聚糖A-50柱(3.5×40cm)上,用含0.07～0.45mol/L NaCl 的上述缓冲液进行线性洗脱。Cal-D 活性被洗脱到最后较小的组份内。把含活性
Vitamin D-dependent calcium-binding protein (Calbindin ~ D, Cal-D) is a cell type calcium-binding protein in a. To distinguish between molecular weight, 28KDa and 10KDa two types. The authors used enzymatic assays to determine the content of 28 kDa Cal-D in human tissues and body fluids. Methods: Human blunt blunt 28KDa Cal-D: normal human kidney homogenate was cooled, repeated centrifugation, the supernatant was heat-precipitated with 0.02mol / L pH7.4Tris-HCl buffer (containing 1mmol / L EDTA, 0.07mol / L NaCl, 0.01% 2-mercaptoethanol) 4 ℃ dialysis solution. Then applied to a DEAE-Dextran A-50 column (3.5 x 40 cm) equilibrated with the above buffer and linearly eluted with the above buffer containing 0.07 to 0.45 mol / L NaCl. Cal-D activity is eluted into the last smaller components. Containing activity