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为了构建抗乙型肝炎病毒（HBV）持续性感染的特异性免疫治疗剂，我们曾构建了人白细胞介素-2（IL－2）与HBV前S（preS）抗原的融合蛋白（IL－2preS）的真核表达克隆，并证明IL－2preS融合蛋白能在哺乳动物细胞中分泌表达。本文以该克隆为基因疫苗，经正常肌肉组织内接种和再生性肌肉组织内接种进行基因免疫，实验结果证明接种IL－2preS基因疫苗能在动物体内诱导抗preS抗体产生，免疫后15天可检出抗体，45天时达高峰值，60天不见下降。再生性肌肉内的免疫效果比正常肌肉的好，所产生的抗体水平前者显著高于后者。结果表明IL－2pres融合蛋白能在机体内分泌表达。提示IL－2preS基因疫苗在人体内可能有多重生物功能，如诱导体液免疫与细胞免疫应答，双重导向作用和细胞膜受体交联效应等。
To construct a specific immunotherapeutic against hepatitis B virus (HBV) persistent infection, we have constructed a fusion protein of human interleukin-2 (IL-2) and HBV preS antigen (IL-2preS ), And demonstrated that IL-2preS fusion protein can be secreted and expressed in mammalian cells. In this paper, the cloned gene vaccine, the normal muscle tissue inoculation and regenerative muscle tissue inoculation for gene immunization, the experimental results show that inoculation of IL-2preS gene vaccine can induce anti-preS antibodies in animals, 15 days after immunization can be seized Antibodies, peaked at 45 days, but not decreased at 60 days. Regenerative muscle immune effects than normal muscles, the level of antibodies produced by the former was significantly higher than the latter. The results show that IL-2pres fusion protein can be secreted in the body. The results suggest that the IL-2preS gene vaccine may have multiple biological functions in human body, such as inducing humoral and cellular immune responses, double-oriented and cell-membrane receptor cross-linking effects.