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目的研究8周的运动和膳食等生活方式干预对胰岛素抵抗大鼠氧化应激的影响。方法采用高脂饲料建立肥胖诱导的胰岛素抵抗大鼠模型,以体重和脂体比作为肥胖程度的衡量标准,以口服糖耐量试验葡萄糖曲线下面积作为糖耐量衡量标准。再分别进行8周的中等强度跑台运动、相对低脂的基础饲料及两者联合干预,以铜试剂法测定血清游离脂肪酸(FFA)含量,以黄嘌呤氧化酶法和硫代巴比妥酸法测定肝脏、脂肪组织和比目鱼肌中超氧化物歧化酶(SOD)活性与丙二醛(MDA)含量,以FFA含量和SOD/MDA衡量大鼠氧化应激状态。结果 8周干预后,3种干预均降低了大鼠体重(P<0.01)、脂体比(P<0.01)和葡萄糖曲线下面积(P<0.01)。膳食和联合干预降低了血清FFA浓度(P<0.01),单独的运动干预没有这种效应。3种干预提高了肝脏和脂肪组织中SOD/MDA(P<0.01),运动和联合干预提高了比目鱼肌中的SOD/MDA(P<0.05),而单独的膳食干预没有这种效应。结论运动和膳食干预能改善大鼠的胰岛素抵抗状态,这与氧化应激状态的缓解有关。
Objective To study the effects of 8-week lifestyle and dietary lifestyle intervention on oxidative stress in insulin resistant rats. Methods The rat model of obesity-induced insulin resistance was established by using high-fat diet. The body weight and lipid ratio were taken as the measure of obesity. The area under the curve of oral glucose tolerance test was taken as the standard of glucose tolerance. Then the rats were subjected to moderate intensity treadmill exercise for 8 weeks, relative low-fat diet and the combination of the two. The contents of free fatty acids (FFA) in serum were determined by the method of copper reagent. The contents of serum free fatty acids (FFA) were determined by xanthine oxidase method and thiobarbituric acid Method was used to determine the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver, adipose tissue and soleus muscle. The oxidative stress was measured by FFA and SOD / MDA. Results After 8 weeks of intervention, the body weight (P <0.01), body fat ratio (P <0.01) and area under the curve of glucose (P <0.01) were decreased in the three intervention groups. Dietary and combined interventions decreased serum FFA concentrations (P <0.01), with no effect of exercise alone. The three interventions increased SOD / MDA in liver and adipose tissue (P <0.01). Exercise and combined intervention increased SOD / MDA in soleus muscle (P <0.05), but no effect was observed in dietary intervention alone. Conclusion Exercise and dietary intervention can improve insulin resistance in rats, which is related to the alleviation of oxidative stress.