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目的探讨内源性硫化氢(H2S)及其合成酶胱硫醚-β-合成酶(CBS)、胱硫醚-γ-裂解酶(CSE)和3-巯基丙酮酸硫转移酶(MPST)在正常膀胱组织以及膀胱癌中的表达。方法选取因膀胱癌而行膀胱肿瘤电切术或膀胱癌根治术的肿瘤组织标本76例,同时收集15例正常膀胱组织作对照组。通过免疫组化和Western blot研究CBS、CSE和MPST在正常膀胱组织以及不同病理分期/分级膀胱癌组织中的表达情况;通过敏感硫电极法检测H2S在正常膀胱组织和膀胱癌中的含量。分别比较H2S及其合成酶在各组之间的差异,初步探讨H2S及其合成酶与膀胱癌恶性进展之间的关系。结果免疫组化结果显示CBS(79.1%)、CSE(81.3%)和MPST(84.6%)在膀胱癌黏膜和肌层组织中均有表达。CBS(P=0.021和P<0.001)和MPST(P=0.015和P=0.045)的表达在膀胱癌不同分期和分级之间有统计学差异。在所有检测的标本中,肌层浸润性膀胱癌中的H2S含量最高[(52.6±2.91)nmol·mg-1·min-1],各组之间有统计学差异。结论内源性H2S及其合成酶CBS、CSE和MPST在膀胱癌组织中的表达明显高于正常膀胱组织,且其表达在膀胱癌不同分期和分级之间有统计学差异。
Objective To investigate the effects of endogenous hydrogen sulfide (H2S) and its synthetic enzymes cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) and 3-mercaptopyruvate sulfotransferase Normal bladder tissue and bladder cancer. Methods Sixty-six cases of bladder cancer with bladder cancer resection or radical resection of bladder cancer were selected, and 15 cases of normal bladder tissue were collected as control group. The expression of CBS, CSE and MPST in normal bladder tissues and in different pathological staging / grading bladder cancer tissues was studied by immunohistochemistry and Western blot. The content of H2S in normal bladder tissue and bladder cancer was detected by sensitive sulfur electrode method. The differences of H2S and its synthase in each group were compared respectively, and the relationship between H2S and its synthase and malignant progression of bladder cancer was discussed. Results The results of immunohistochemistry showed that CBS (79.1%), CSE (81.3%) and MPST (84.6%) were expressed in both mucosa and muscular tissue of bladder cancer. The expression of CBS (P = 0.021 and P <0.001) and MPST (P = 0.015 and P = 0.045) were statistically different between different stages and grading of bladder cancer. Among all the specimens tested, the content of H2S in myometrial invasive bladder cancer was the highest [(52.6 ± 2.91) nmol · mg-1 · min-1] with statistical significance. Conclusions The expressions of endogenous H2S and its synthetic enzymes CBS, CSE and MPST in bladder cancer tissues were significantly higher than those in normal bladder tissues, and their expressions were significantly different between different stages and grading of bladder cancer.