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1973年Latt用含有5-溴脲嘧啶脱氧核苷(Brdu)的培养基培养人的淋巴细胞染色体,然后用荧光染料染色,使两条姐妹染色单体发出强弱不同的荧光,建立了姐妹染色单体交换(SCE)技术。次年Perry和Korenberg改进了这一技术,用Giemsa代替荧光染
In 1973, Latt was used to culture human lymphocyte chromosomes with medium containing 5-bromouridine (Brdu) and then stained with a fluorescent dye to make two sister chromatids give different intensities and different fluorescences to establish a sister stain Monomer exchange (SCE) technology. The following year Perry and Korenberg improved this technique by replacing Giemsa with fluorescent stains