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克虫磷(Curacron)是一种广泛应用于棉花地的有机磷杀虫剂。为了检测其对哺乳动物生殖细胞的诱变性,作者分析了经克虫磷染毒小鼠的精原细胞染色体畸变和精子异常情况,包括精子形态、精子活性和精子数量。实验采用雄性瑞士小鼠,体重25～30g,管饲法给药。精原细胞染色体畸变分析实验中分三个剂量给药,即12、36和72mg/kg,相当于LD_(50)的1/24、1/8和1/4,用急性(一次给药)和亚急性(连续五天)两种染毒方法,取一未经染毒组作对照。急性染毒后24小时和48小时后处死动物,处死前4小时腹腔注射10mg/kg秋水仙素,制
Curacron is a organophosphate insecticide widely used in cotton fields. In order to test its mutagenicity in mammalian germ cells, the authors analyzed the chromosomal aberrations and sperm abnormalities in spermatozoa of mice exposed to acrinomycin, including sperm morphology, sperm motility and sperm count. Experimental male Swiss mice weighing 25 ~ 30g, gavage administration. Chromosome aberration analysis of spermatogonia experiments were divided into three doses, namely 12,36 and 72mg / kg, which is equivalent to LD50 (50) 1 / 24,1 / 8 and 1/4, with acute (one dose) And subacute (five consecutive days) two methods of exposure, take a non-exposed group as a control. Animals were sacrificed at 24 hours and 48 hours after acute exposure and 10 mg / kg colchicine 4 hours prior to sacrifice