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目的:建立昆丹胶囊中芍药内酯苷、芍药苷、二苯乙烯苷、五没食子酰葡萄糖的含量测定方法。方法:采用Agilent TC-C18柱(4.6 mm×250 mm,5μm),以乙腈-甲醇-0.1%磷酸为流动相进行梯度洗脱,检测波长230 nm,柱温30℃,流速1.0 mL.min-1。结果:芍药内酯苷、芍药苷、2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷及五没食子酰葡萄糖浓度分别在0.02～1.84,0.03～1.65,0.03～3.44,0.01～0.81 mg(r>0.999 9)线性关系良好。低、中、高3个浓度的平均加样回收率分别为99.5%～102.6%(RSD 1.3%～2.1%),98.5%～103.5%(RSD 0.5%～2.4%),98.3%～104.2%(RSD 0.5%～2.2%)。结论:方法准确,灵敏,能有效测定昆丹胶囊中芍药内酯苷、芍药苷、2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷及五没食子酰葡萄糖成分的含量。
OBJECTIVE: To establish a method for the determination of the content of paeoniflorin, paeoniflorin, stilbene glycoside, and galloylglucose in Kun Dan capsule. METHODS: The mobile phase was eluted with acetonitrile-methanol-0.1% phosphoric acid gradient on an Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) with a detection wavelength at 230 nm and a column temperature of 30 ° C at a flow rate of 1.0 mL · min- 1. Results: The concentrations of paeoniflorin, paeoniflorin, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside and pentagalloylglucose were 0.02 ~ 1.84, 0.03 ~ 1.65 , 0.03 ~ 3.44,0.01 ~ 0.81 mg (r> 0.999 9). The average recoveries of low, medium and high concentrations were 99.5% -102.6% (RSD 1.3% -2.1%), 98.5% -103.5% (RSD 0.5% -2.4%), 98.3% -104.2% RSD 0.5% ~ 2.2%). Conclusion: The method is accurate and sensitive and can be used to determine the contents of paeoniflorin, paeoniflorin, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, The content of acyl glucose components.