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目的 :探讨FSH对人腔前卵泡 (preantralfollicle)体外生长的影响。方法 :培养液中加入不同浓度的FSH ,观察卵泡存活天数和卵泡发育增大。结果 :卵泡体外存活天数 :对照组为 2 .80± 1.6 9天 ,Ⅰ、Ⅱ、Ⅲ组 (FSH浓度为 0 .5IU/ml、1IU/ml、2IU/ml)分别为 5 .36± 0 .6 3、5 .47± 2 .5 0、8.13± 4.19天 ,与对照组相比 ,分别为P <0 .0 5、P <0 .0 1、P <0 .0 0 1。卵泡增大比率 :对照组为 2 0 .0 0 % ,Ⅰ、Ⅱ、Ⅲ组分别为 35 .71%、6 0 .0 0 %、81.2 5 % ,与对照组相比分别为P >0 .0 5、P <0 .0 5、P <0 .0 1。结论 :FSH能使卵泡体外存活天数延长 ,卵泡发育增大 ,并大致呈正相关关系。
Objective: To investigate the effect of FSH on the growth of preantral follicles in vitro. Methods: Different concentrations of FSH were added into the culture medium to observe the survival time of follicles and the growth of follicles. Results: The survival time of follicles in vitro was 2.80 ± 1.6 days in the control group, and it was 5.36 ± 0 in the groups Ⅰ, Ⅱ and Ⅲ (the FSH concentration was 0.5 IU / ml, 1 IU / ml and 2 IU / ml). 6 3,5 .47 ± 2 .5 0,8.13 ± 4.19 days, respectively, compared with the control group, P <0.05, P <0.01, P <0.001. The rate of follicular enlargement was 20.0% in control group, 35.71%, 60.0% and 81.2% respectively in group Ⅰ, Ⅱ and Ⅲ, which were respectively P> 0 compared with control group. 0 5, P <0 .0 5, P <0 .0 1. Conclusion: FSH can prolong the number of follicles in vitro survival, follicular development increased, and generally positive correlation.