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目的:探讨叶酸-壳聚糖Prdx6 shRNA纳米粒对胃癌细胞生长的影响。方法:制备靶向性叶酸-壳聚糖Prdx6 shRNA纳米粒,原子力显微镜观察其形态,激光粒度分析仪测定纳米粒的粒径;倒置荧光显微镜观察叶酸-壳聚糖Prdx6 shRNA纳米粒的转染效率;采用蛋白质印迹法检测胃癌细胞Prdx6蛋白的表达变化;CCK8细胞增殖实验检测胃癌细胞的存活率。结果:1制备成功叶酸-壳聚糖Prdx6 shRNA向纳米粒。2荧光显微镜下观察靶向性叶酸-壳聚糖Prdx6 shRNA纳米粒转染胃癌细胞的效率明显高于非靶向纳米粒;胃癌细胞转染靶向组纳米粒后Prdx6蛋白的表达显著低于非靶向组。3与对照组相比,叶酸-壳聚糖Prdx6 shRNA纳米粒能够明显抑制胃癌细胞的增殖(P<0.01)。结论:1叶酸-壳聚糖Prdx6 shRNA纳米粒可高效转染胃癌细胞。2转染叶酸-壳聚糖Prdx6 shRNA纳米粒后胃癌细胞的生长明显受抑制。
Objective: To investigate the effect of folic acid-chitosan Prdx6 shRNA nanoparticles on the growth of gastric cancer cells. Methods: Targeted folic acid-chitosan Prdx6 shRNA nanoparticles were prepared. Morphology was observed with atomic force microscopy. The particle size of the nanoparticles was measured by laser particle size analyzer. The transfection efficiency of folic acid-chitosan Prdx6 shRNA nanoparticles was observed by inverted fluorescence microscope The expression of Prdx6 protein in gastric cancer cells was detected by Western blotting. The survival rate of gastric cancer cells was detected by CCK8 cell proliferation assay. Results: 1 Preparation of folic acid-chitosan Prdx6 shRNA to nanoparticles. The results showed that the efficiency of Prdx6 shRNA nanoparticles transfected into gastric cancer cells was significantly higher than that of non-targeted nanoparticles. The expression of Prdx6 protein in gastric cancer cells transfected with targeting nanoparticles was significantly lower than that of non-targeted nanoparticles Targeted group. Compared with the control group, folic acid-chitosan Prdx6shRNA nanoparticles can significantly inhibit the proliferation of gastric cancer cells (P <0.01). Conclusion: 1 folic acid-chitosan Prdx6 shRNA nanoparticles can efficiently transfect gastric cancer cells. 2 transfected with folic acid-chitosan Prdx6 shRNA nanoparticles significantly inhibited the growth of gastric cancer cells.