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将18只豚鼠随机分为3组,采用豚鼠最大值试验法(GPMT),设立TCE实验组、阴性对照组、阳性对照组,用皮内注射的方式分别注射三氯乙烯(TCE)、橄榄油、24-二硝基氯苯(DNCB)。并收集豚鼠外周血和脾淋巴细胞,用Real-time荧光定量PCR检测凋亡基因表达水平。TCE实验组和阳性对照组动物出现明显皮肤损害。TCE实验组和阳性对照组脾淋巴细胞Bax、Bad、Bcl-2的mRNA表达水平比阴性对照组显著升高(P<0.01);但TCE实验组和阳性对照组外周血淋巴细胞Bax、Bad、Bcl-2的mRNA表达水平与阴性对照组比较差异无统计学意义(P>0.05)。提示三氯乙烯可诱导豚鼠产生明显的迟发型皮肤变态反应,致豚鼠脾淋巴细胞凋亡基因表达水平发生明显改变。
Twenty-eight guinea pigs were randomly divided into three groups: TCE experimental group, negative control group and positive control group were established by guinea pig maximum test method (GPMT). TCE and olive oil were injected intradermally , 24-dinitrochlorobenzene (DNCB). The guinea pig peripheral blood and spleen lymphocytes were collected and the expression of apoptosis gene was detected by Real-time fluorescent quantitative PCR. TCE experimental group and positive control animals showed obvious skin lesions. The mRNA expressions of Bax, Bad and Bcl-2 in spleen lymphocytes of TCE experimental group and positive control group were significantly higher than those in negative control group (P <0.01). However, the levels of Bax, Bad, The mRNA expression level of Bcl-2 was not significantly different from that of the negative control group (P> 0.05). Tip trichlorethylene can induce guinea pigs produce significant delayed type skin allergy, guinea pig spleen lymphocyte apoptosis gene expression levels have changed significantly.