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石灰小体是绦虫纲虫体内的重要组织成分,猪囊尾蚴体内也很多见,可视为一个诊断猪囊虫病的指标。作者在酸卡红及硝酸银染色的基础上对试荊浓度和染色时间加以改良,并作了对比,现报告如下。 1 材料和方法取刚刚屠宰、自然感染猪囊尾蚴病的猪肌肉内的囊尾蚴,用生理盐水反复冲洗后,放入40％胆汁生理盐水培养皿内,37℃温箱温育,待头节伸出后,再用水冲洗,用2张载玻片把囊尾蚴头节压扁,压片用5％的甲醛溶液固定20小时,待用。酸卡红染色法:取囊尾蚴头节,洗去固定液,用酒精梯度(30％、50％、60％、70％、80％)脱水各5分钟。酸卡红染色20～30小
Lime body is an important tissue component in the body of tapeworm, Cysticercus cellulosae are also seen in vivo, can be regarded as an indicator of diagnosis of swine cysticercosis. The authors in the acid red and silver nitrate stain on the basis of the test Jing concentration and dyeing time be improved, and made a comparison, are as follows. 1 Materials and methods Take freshly slaughtered, naturally infected cysticercosis in pig muscle cysticerci, washed repeatedly with saline, placed in 40% bile saline dish, incubated at 37 ℃ incubator until the head section After reaching out, rinse again with water, use 2 slides to compress cysticercosis head section, tablet with 5% formaldehyde solution for 20 hours, stand by. Acid card red staining: cysticercus first section, wash away the fixative, dehydration with alcohol gradient (30%, 50%, 60%, 70%, 80%) for 5 minutes. Acid card red staining 20 to 30 small