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用高压液相色谱法研究了豫麦18(抗旱性较强)和扬麦9号(抗旱性较弱)小麦胚芽鞘中三种不同形态的多胺(polyamine,PA):游离态多胺(PA)、高氯酸可溶性结合态多胺(PS结合态PA)和高氯酸不溶性结合态多胺(PIS 结合态PA)与渗透胁迫的关系。结果发现:渗透胁迫2d,豫麦18胚芽鞘中的游离态Spd和游离态Spm 的含量明显上升,而扬麦9 号的游离态Put 的上升明显。S- 腺苷蛋氨酸脱羧酶(S-AMDC)的抑制剂——甲基乙二醛-双(鸟嘌呤腙)(MGBG)处理豫麦18,明显抑制了渗透胁迫诱导的游离态Spd 和游离态Spm 的增加,并且加重了渗透胁迫伤害,外源Spd处理扬麦9号明显促进了渗透胁迫诱导的游离态Spd 和游离态Spm 的增加,并且减缓了渗透胁迫的伤害。渗透胁迫下,豫麦18 胚芽鞘中的PS 结合态PA 和PIS结合态PA 的上升幅度都明显大于扬麦9号。菲咯啉(o-Phen)抑制渗透胁迫下PIS 结合态PA的合成并加重了渗透胁迫对胚芽鞘的伤害。这些结果表明:小麦胚芽鞘中的游离态Spd、游离态Spm、PS 结合态PA 和PIS 结合态PA 的升高有利于增强渗透胁迫抗性。
Three different forms of polyamine (PA) in wheat germ sheath of Yumai 18 (with stronger drought resistance) and Yangmai 9 (less drought tolerant) were studied by high pressure liquid chromatography (HPLC): free polyamine ), Perchloric acid soluble bound polyamine (PS bound PA) and perchloric acid insoluble bound polyamine (PIS bound PA) and osmotic stress. The results showed that the contents of free Spd and free Spm in the coleoptile of Yumai 18 significantly increased 2 days after osmotic stress, while that of Yangmai 9 increased significantly. Treatment of Yumai 18 with S-adenosylmethionine decarboxylase (S-AMDC) inhibitor methylglyoxal-bis (guanylhydrazone) (MGBG) significantly inhibited osmotic stress-induced free Spd and free Spm Increased exacerbated by osmotic stress, exogenous Spd treatment Yangmai 9 significantly promoted the osmotic stress-induced increase of free Spd and free Spm, and slow down the damage of osmotic stress. Under osmotic stress, the increase of PS-bound PA and PIS-bound PA in Yumai 18 coleoptile was significantly greater than that of Yangmai 9. Phenanthroline (o-Phen) inhibits the synthesis of PIS-bound PA under osmotic stress and aggravates the injury of coleoptile by osmotic stress. These results indicate that the increase of free Spd, free Spm, PS-bound PA and PIS-bound PA in wheat coleopathies is beneficial to increase osmotic stress resistance.