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目前毛细管色谱的应用中,对耐高温极性的毛细管柱的需要愈来愈多,而一般用物理涂渍方法制备的毛细管柱,在高温时常由于固定液的流失和液膜的破裂形成液珠而致损坏。Madani等和Blamberg等曾将一些固定液化学键合到玻璃毛细管的内壁上,得到热稳定性较好的液相涂层。我们用有机胶为粘合剂,在玻璃毛细管内壁涂敷细颗粒(2-3μ)的硅胶或白色405担体制成PLOT柱,然后将甲基苯基聚硅氧烷化学键合到担体涂层上。 SCOT columns of good thermal stability with slightly polar stationary phase were prepared by chemically bonding the porous layer(such as 405 white diatomaceous support and silica gel)of capillary column with methyl phenyl polysiloxane. Such columns show good efficiency and nearly no bleeding at 300℃. Satisfactory results were obtained in the use of these columns for analyzing polynuclear aromatic hydrocarbons and saturated isoprenoid hydrocarbon(such as pristare/C_(17)H_(36) and phytane/C_(18)H_(38) pairs).
At present, capillary column chromatography requires more and more capillary column with high temperature polarity. However, capillary columns prepared by physical coating method usually form liquid droplets due to the loss of the fixing liquid and the rupture of the liquid film at high temperature Damage caused. Madani et al. And Blamberg et al. Have chemically bonded some of the immobilized liquid to the inner walls of glass capillaries to obtain a liquid coating with better thermal stability. We use organic glue as a binder to coat the inside of the glass capillary with fine particles (2-3μ) of silica gel or white 405 support to make a PLOT column, and then chemically bond the methylphenylpolysiloxane to the support coating . SCOT columns of good thermal stability with slightly polar stationary phase were prepared by chemically bonding the porous layer (such as 405 white diatomaceous support and silica gel) of capillary column with methyl phenyl polysiloxane. Such columns show good efficiency and nearly no bleeding at 300 ° C Satisfactory results were obtained in the use of these columns for analyzing polynuclear aromatic hydrocarbons and saturated isoprenoid hydrocarbons (such as pristine / C_ (17) H_ (36) and phytane / C_ (18) H_ (38) pairs).