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目的旨在了解折肢疼痛信息传递的途径与参与氟烷麻醉作用的中枢部位。方法用ＳＤ大鼠１６只，制造双后肢折断刺激和氟烷麻醉１小时，应用免疫组织化学法，光镜观察ｆｏｓ蛋白阳性神经元在中枢的分布。结果折肢组在外侧隔核、丘脑室旁核、下丘脑室旁核腹侧部、视上核和脊髓后角等１１个核团出现ｆｏｓ蛋白阳性神经元。氟烷吸入麻醉组和折麻组在杏仁核簇、终纹床核、海马Ⅰ区、丘脑中央中核、内外侧缰核、外侧隔核、中视前核、丘脑室旁核、下丘脑室旁核腹侧部、中脑导水管周围灰质和视上核等２４个核团出现ｆｏｓ蛋白阳性神经元，主要分布在端脑、间脑和脑干。结论疼痛刺激和氟烷吸入麻醉在中枢存在着部分相同的调控部位
The aim is to understand the pathway of limb pain delivery and the central part involved in halothane anesthesia. Methods Sixteen Sprague-Dawley rats were used to make double hindlimb fracture stimulation and halothane anesthesia for 1 hour. Immunohistochemistry and light microscope were used to observe the distribution of fos protein positive neurons in the central nervous system. Results In the limbs group, fos protein positive neurons appeared in 11 nuclei such as lateral septal nucleus, paraventricular nucleus of hypothalamus, ventral part of paraventricular nucleus of hypothalamus, supraoptic nucleus and posterior horn of spinal cord. Hyaluronan inhalation anesthesia group and crease group in the amygdala cluster, terminal striation bed nucleus, hippocampus Ⅰ area, central thalamic nucleus, medial and lateral habenular nucleus, lateral septal nucleus, the preoptic nucleus, hypothalamic paraventricular nucleus, hypothalamic paraventricular nucleus Fos protein positive neurons were found in 24 nuclei such as ventral part, periaqueductal gray and supraoptic nucleus, mainly distributed in telencephalon, diencephalon and brainstem. Conclusions There is some of the same regulatory sites in the center of pain stimulation and halothane inhalation anesthesia