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目的:研究复方薤白胶囊对过氧化氢(H2O2)诱导的凋亡内皮细胞的保护作用,及相关凋亡基因表达的影响,以明确其作用机制。方法:体外培养人脐静脉内皮细胞(HUVEC),以H2O2行内皮细胞凋亡造模后,再加入不同浓度的复方薤白胶囊提取液作用24h。以MTT法检测细胞增殖率,Hoechst染色观察细胞凋亡,流式细胞仪检测凋亡率,Western Blot检测抗凋亡基因Bcl-2含量变化。结果:H2O2造模后,细胞增殖明显受抑,Hoechst染色见大量凋亡细胞,流式细胞检测出明显凋亡峰,抗凋亡基因Bcl-2含量降低,与正常组相比P<0.01,具有明显的统计学意义。而各中药组提高了造模后细胞增殖率,降低了凋亡细胞及凋亡峰,提高了Bcl-2含量表达,且与浓度呈正相关,具有明显的统计学意义。结论:复方薤白胶囊能上调抗凋亡基因Bcl-2的表达,保护内皮细胞,抗内皮细胞凋亡。
Objective: To investigate the protective effect of compound Qibai Capsule on hydrogen peroxide (H2O2)-induced apoptotic endothelial cells, and the effect of related apoptotic gene expression, in order to clarify its mechanism of action. METHODS: Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and after endothelial cell apoptosis was induced with H2O2, different concentrations of compound Qibai Capsule extract were added for 24 hours. The cell proliferation rate was detected by MTT assay, apoptosis was observed by Hoechst staining, the apoptosis rate was detected by flow cytometry, and the anti-apoptotic gene Bcl-2 content was detected by Western Blot. RESULTS: After H2O2 production, cell proliferation was significantly inhibited. A large number of apoptotic cells were stained with Hoechst staining. Apoptotic peaks were detected in flow cytometry, and the anti-apoptotic gene Bcl-2 content was decreased. P<0.01 compared with the normal group. There is a significant statistical significance. The Chinese medicine group increased cell proliferation rate after modeling, decreased apoptotic cells and apoptotic peaks, increased the expression of Bcl-2, and was positively correlated with the concentration, showing significant statistical significance. Conclusion: Compound Qibai Capsule can up-regulate the expression of anti-apoptosis gene Bcl-2, protect endothelial cells and resist apoptosis of endothelial cells.