Brain-derived neurotrophic factor induces neuron-like cellular differentiation of mesenchymal stem c

• 作 者: Lei Chen Zhongguo Zhang Bing Chen Xiaozhi Liu Zhenlin Liu Hongliang Liu Gang Li Zhiguo Su Junfei Wang Guozhen Hui
• 机 构: Department of Neurosurgery, Tianjin Fifth Central Hospital, Tianjin 300450, China“,”Department of Co
• 出 处: 中国神经再生研究（英文版）
• 发表日期: 2011年13期
• 关键词: human umbilical cord blood purification and culture brain-derived neurotrophic f

Human umbilical cord blood was collected from full-term deliveries scheduled for cesarean section. Mononuclear cells were isolated, amplified and induced as mesenchymal stem cells. Isolated mesenchymal stem cells tested positive for the marker CD29, CD44 and CD105 and negative for typical hematopoietic and endothelial markers. Following treatment with neural induction medium containing brain-derived neurotrophic factor for 7 days, the adherent cells exhibited neuron-like cellular morphology. Immunohistochemical staining and reverse transcription-PCR revealed that the induced mesenchymal stem cells expressed the markers for neuron-specific enolase and neurofilament. The results demonstrated that human umbilical cord blood-derived mesenchymal stem cells can differentiate into neuron-like cells induced by brain-derived neurotrophic factor in vitro.