论文部分内容阅读
目的 探讨乙型肝炎血清学 5项指标与乙型肝炎病毒 - DNA(HBV- DNA)含量的关系 ,了解乙肝血清免疫学指标的各种组合模式的病毒含量情况 ,以此估计其传染性程度 ,为乙肝的预防、诊断和治疗提供实验室依据。方法 用美国 Abbott公司的 AXSYM全自动免疫分析系统和微粒子酶免疫检测试剂盒 ,检测血清乙型肝炎 5项指标 ;根据结果选出常见 6种组合模式 ,每种组合随机取样 5 0例 ,共 30 0例 ;并以聚合酶链反应 (PCR)定量检测血清 HBV- DNA含量。结果 在各种组合模式中 ,均能不同程度地检出 HBV- DNA,在乙肝表面抗原阳性标本其HBV- DNA含量明显高于乙肝表面抗原阴性的标本 ,其中以乙肝表面抗原 (HBs Ag) (+) /乙肝 e抗原 (HBe Ag)(+) /乙肝核心抗体 (HBc Ab) (+)形式病毒含量最高 (大于 10 6拷贝 / μl,占 84% ) ,阳性率为 10 0 % ;而乙肝表面抗体阳性的组合模式 ,HBV- DNA阳性率较低 ,且含量在 10 6拷贝 / μl以下。结论 血清乙肝 5项标志阳性者 ,无论何种组合模式均存在病毒复制的可能
Objective To investigate the relationship between hepatitis B serological 5 indexes and hepatitis B virus DNA (DNA-DNA) content and to understand the virus content of various combination modes of hepatitis B serum immunological indexes to estimate the degree of infectivity, Provide a laboratory basis for the prevention, diagnosis and treatment of hepatitis B. Methods Abbott’s AXSYM automated immunoassay system and microparticle enzyme immunoassay kit were used to detect five indicators of hepatitis B. Five common patterns of hepatitis B were selected according to the results of random sampling of 50 cases 0 cases. Polymerase chain reaction (PCR) was used to quantitatively detect serum HBV DNA content. Results HBV DNA was detectable to various degrees in all combinations, and the content of HBV DNA in HBsAg-positive specimens was significantly higher than that in HBsAg-negative specimens. Among them, HBsAg (+) / HBeAg (+) / HBc Ab (+) form had the highest virus content (over 106 copies / μl, accounting for 84%) with a positive rate of 10% Surface antibody-positive combination mode, HBV-DNA positive rate is low, and the content of 10 6 copies / μl below. Conclusions Serum hepatitis B five markers were positive, no matter what combination mode there is the possibility of viral replication