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采用电子鼻zNose~(TM)对棉花植株常见挥发性化合物进行定性分析,确定每类化合物的相对保留指数,构建棉花植株常见挥发性化合物特征谱库.采集棉铃虫幼虫取食诱导棉株挥发性化合物,利用电子鼻构建棉铃虫幼虫危害棉株的指纹图谱.比对健康棉株与棉铃虫幼虫危害棉株的色谱图及指纹图谱发现,棉铃虫幼虫危害棉株的挥发物的种类和含量均比健康棉株的多,而且未去除棉铃虫幼虫的危害处理与去除棉铃虫幼虫的危害处理相比,被诱导挥发物的含量显著升高.通过电子鼻实时监测棉花植株各处理β-蒎烯、β-月桂烯等挥发性化合物的释放节律.结果显示,当棉铃虫幼虫危害棉株2h后,β-蒎烯的释放量明显增加,与对照相比差异显著.β-月桂烯在棉铃虫幼虫危害2h的释放量与对照相比没有显著差异,在棉铃虫幼虫危害24h释放量显著增加.6～8h β-蒎烯和β-月桂烯分别达到各自最大释放量,随后缓慢回落,但仍然保持较高释放水平.图3表1参24
The electronic nose zNose ~ (TM) was used to qualitatively analyze the common volatile compounds of cotton plants, and the relative retention index of each compound was determined to construct the common spectrum of volatile compounds in cotton plants. Compounds, the use of electronic nose to build cotton bollworm larvae harm cotton fingerprinting.Comparison of healthy cotton plants and cotton bollworm larvae harm cotton strains of the chromatogram and fingerprinting found that the cotton bollworm larvae damage cotton volatiles species and content were Compared with healthy cotton plants, the damage of cotton bollworm larvae was significantly higher than that of cotton bollworm larvae, and the content of volatile compounds was significantly increased in cotton bollworm by monitoring the changes of β-pinene , Β-myrcene and other volatile compounds.The results showed that when the cotton bollworm larvae damage cotton plants 2h, the release of β-pinene significantly increased, compared with the control significant difference.β-myrcene in the cotton bollworm Larvae 2h release no significant difference compared with the control, the release of the bollworm larvae significantly increased 24h exposure 6 ~ 8h β-pinene and β-myrcene reached their respective Maximum release, then slow down, but still maintain a high level of release.