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目的:骨钙蛋白(BGP)作为骨形成的特异、敏感指标与儿童的生长发育关系密切,该研究即通过测定不同年龄段小儿血清骨钙蛋白的水平来评价儿童成骨活动和骨转换的动态变化规律,并对该规律做进一步的分析。方法:采用酶联免疫吸附分析法(ELISA)测定62例健康儿童(0～3个月为A组13例,～1岁为B组9例,～3岁为C组13例,～9岁为D组14例,9岁以上为E组13例)血清骨钙蛋白含量,并比较不同年龄段小儿血清BGP的水平。结果:A、B、C、D、E组的小儿血清骨钙蛋白水平分别为(21.52±18.69)ng/ml、(41.18±22.69)ng/ml、(32.07±19.94)ng/ml、(35.89±17.10)ng/ml和(69.13±35.64)ng/ml,各组间比较P<0.001(F=23.58),存在统计学差异。结论:①血清骨钙蛋白水平在儿童期与年龄呈正相关r=0.38,P<0.01;即年龄越大,骨钙蛋白的含量越高,总体呈现出上升趋势。②血清骨钙蛋白含量随年龄增长呈现出两个高峰,分别为3月～1岁和9～14岁,说明在婴儿期及青春期成骨细胞活性最高,骨基质的矿化过程最为活跃,这与儿童期的骨代谢特点相吻合,进一步验证了血清骨钙蛋白水平可以作为评价儿童骨骼生长发育的客观指标。
OBJECTIVE: BGP is a specific and sensitive marker of bone formation and is closely related to the growth and development of children. This study evaluated the dynamic changes of osteogenic activity and bone turnover in children by measuring serum osteocalcin levels in children of different ages The law of change, and further analysis of the law. Methods: Totally 62 healthy children were enrolled in this study. Enzyme linked immunosorbent assay (ELISA) was used to detect 62 healthy children (13 cases in group A from 0 to 3 months, 9 cases in group B at 1 year old, 13 cases in group C at 3 years old, 14 cases in group D, and 13 cases in group E as group E). Serum BGP levels were also compared among different age groups. Results: Serum osteocalcin levels of children in groups A, B, C, D and E were (21.52 ± 18.69) ng / ml, (41.18 ± 22.69) ng / ml, ± 17.10) ng / ml and (69.13 ± 35.64) ng / ml, P <0.001 for each group (F = 23.58), there was statistical difference. Conclusion: Serum osteocalcin levels in childhood and age were positively correlated (r = 0.38, P <0.01); that is, the older, the higher the content of osteocalcin, showing an overall upward trend. ② Serum osteocalcin levels showed two peaks with age, ranging from March to 1 year and from 9 to 14 years, indicating that osteoblasts were the most active in infancy and adolescence, and the mineralization of bone matrix was the most active It is consistent with the characteristics of bone metabolism in childhood to further verify the serum osteocalcin levels can be used as an objective evaluation of children’s bone growth and development.