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目的克隆大鼠视网膜缓慢变性／盘膜边缘蛋白（ｒｅｔｉｎａｌｄｅｇｅｎｅｒａｔｉｏｎｓｌｏｗ／ｐｅｒｉｐｈｅｒｉｎ，ＲＤＳ／ｐｅｒｉｐｈｅｒｉｎ）基因。方法以ＳＤ大鼠的视网膜ｐｏｌｙＡ＋ＲＮＡ为模版，采用ＲＴ－ＰＣＲ的方法扩增一段约１５５５ｂｐ的ｃＤＮＡ片段，并亚克隆至ｐＢｌｕｅｓｃｒｉｐｔⅡＫＳ（＋）载体中，进行限制性内切酶酶切和序列分析。结果证实所克隆的片段是大鼠的ＲＤＳ／ｐｅｒｉｐｈｅｒｉｎｃＤＮＡ，与Ｂｅｇｙ等报道的序列［１］相比，除３′端非翻译区的第１２４２位的碱基Ａ→Ｇ，第１４０９～１４１１位碱基ＣＡ→ＣＣＡ外，其它序列基本一致［１］。结论已获得大鼠ＲＤＳ／ｐｅｒｉｐｈｅｒｉｎ基因的ｃＤＮＡ，为研究ＲＤＳ／ｐｅｒｉｐｈｅｒｉｎ基因的功能及在视网膜变性疾病中的作用奠定实验基础。
Objective To clone rat retinal degenerationslow / peripherin (RDS / peripherin) gene. METHODS: A 1555bp cDNA fragment was amplified by RT-PCR from the polyA + RNA of SD rats. The cDNA fragment was subcloned into pBluescriptⅡKS (+) vector for restriction endonuclease digestion and sequence analysis. As a result, it was confirmed that the cloned fragment was RDS / peripherincDNA of rats. Compared with the sequence reported by Begy et al. [1], except for the base A → G at position 1242 in the 3’-untranslated region, bases 1409-1411 Base CA → CCA, other sequences are basically the same [1]. Conclusion The cDNA of rat RDS / peripherin gene has been obtained, which provides experimental basis for studying the function of RDS / peripherin gene and its role in retinal degenerative diseases.