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[目的]探讨舒肝颗粒对酒精性肝纤维化的防治作用及其机制。[方法]用乙醇灌胃的方法制备酒精性肝纤维化大鼠模型。雌性Wistar大鼠随机分为正常对照组、酒精组和舒肝颗粒干预组。实验24周末观察生化和病理改变。[结果]酒精组血清白细胞介素6、肿瘤坏死因子α水平与干预组比较差异有统计学意义(P<0.01)。酒精组Masson胶原染色纤维化较重,而干预组无明显纤维化。24周末,酒精组肝组织中丙二醛与正常组、干预组比较,明显升高(P<0.01);酒精组及干预组肝组织中超氧化物歧化酶活性,明显高于正常组(P<0.05)。SABC免疫组化染色显示24周酒精组肝窦周围及汇管区结蛋白染色阳性物质明显增多,而正常组和干预组仅在汇管区周围有少量阳性物质。[结论]舒肝颗粒具有良好防治大鼠酒精性肝纤维化的作用,其作用机制可能与降低脂质过氧化、抑制肝星状细胞激活转化有关。
[Objective] To explore the preventive and therapeutic effects of Shugan Granule on alcoholic liver fibrosis and its mechanism. [Methods] Alcoholic hepatic fibrosis rat model was established by ethanol gavage. Female Wistar rats were randomly divided into normal control group, alcohol group and Shugan Granule intervention group. Biochemical and pathological changes were observed at the 24th week of the experiment. [Results] The levels of serum interleukin-6 and tumor necrosis factor-α in alcohol group were significantly different from those in the intervention group (P<0.01). Masson collagen staining in the alcohol group was heavier, whereas no fibrosis was observed in the intervention group. At week 24, malondialdehyde in the liver tissue of the alcohol group was significantly higher than that in the normal group and the intervention group (P<0.01). The activity of superoxide dismutase in the alcohol group and the intervention group was significantly higher than that in the normal group (P< 0.05). SABC immunohistochemical staining showed that the number of positive staining for deproteinized proteins around the hepatic sinusoids and the portal tract was significantly increased in the 24-week alcohol group, while there were only a few positive substances around the portal area in the normal group and the intervention group. [Conclusion] Shugan Granules have good effect on prevention and treatment of alcoholic hepatic fibrosis in rats, and its mechanism may be related to reducing lipid peroxidation and inhibiting activation and transformation of hepatic stellate cells.