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目的观察花色苷对心肌细胞损伤的拮抗效应。方法设置3个实验组,即正常对照组,药物损伤组,花色苷组。选取出生1~3 d的SD大鼠30只,将培养48 h的心肌细胞制备成24瓶进行实验,随机分成3组。正常对照组不加任何药物,药物损伤组加入阿霉素ADM 5 mg/ml一共培养,花色苷组加入阿霉素60 min后加入10 mg/L的花色苷。心肌细胞传代培养,测定各相关生化指标。结果与正常对照组比较,药物损伤组心肌肌钙蛋白T(cTnT)、心肌肌钙蛋白Ⅰ(cTnⅠ)、肌酸激酶(CK-MB)明显增高,药物损伤组总超氧化歧化酶(SOD)、CuZn-超氧化歧化酶(CuZn-SOD)和Mn-超氧化歧化酶(Mn-SOD)水平均明显下降(P<0.01);药物损伤组丙二醛(MDA)和NO含量明显增高(P<0.01);而花色苷组上述指标均有改善(P<0.01),与正常对照组比较无统计学意义。结论花色苷对阿霉素造成的心肌损伤具有保护作用。
Objective To observe the antagonistic effect of anthocyanins on cardiomyocyte injury. Methods Three experimental groups were set up, namely normal control group, drug injury group and anthocyanin group. Thirty SD rats aged 1 ~ 3 days were selected, and 24 hours of culture of 48 hours cardiomyocytes were made into 24 bottles and were randomly divided into 3 groups. The normal control group without any drug, the drug injury group added adriamycin ADM 5 mg / ml a total of training, anthocyanin group added doxorubicin 60 min after the addition of 10 mg / L of anthocyanins. Myocardial cell subculture, the determination of the relevant biochemical indicators. Results Compared with the normal control group, the levels of cardiac troponin T (cTnT), cardiac troponin Ⅰ (cTnⅠ) and creatine kinase (CK-MB) were significantly increased in the drug-induced injury group. The total superoxide dismutase (SOD) , CuZn-SOD and Mn-SOD decreased significantly (P <0.01), MDA and NO in the drug-induced injury group were significantly increased (P <0.01). However, the anthocyanin group had the above indexes improved (P <0.01), but no significant difference compared with the normal control group. Conclusion Anthocyanins have a protective effect on myocardial injury caused by doxorubicin.