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利用二氧化硅(SiO_2)纳米微球为模板制备能够装载DNA的微胶囊。首先,利用硅烷偶联剂在SiO_2表面引入氨基吸附DNA,氨基能使SiO_2与DNA之间的静电吸引力增强;继而通过静电吸引力逐层交替吸附聚乙烯亚胺(PEI)和聚丙烯酸(PAA)进行层层自组装,形成以SiO_2纳米微球为模板的包覆多层高分子的载DNA微球,利用Zeta电位测试逐层吸附后微球表面的电位来确定组装的进行。溶出SiO_2后,可以获得载DNA的多层高分子材料包覆的微胶囊。最后通过透射电子显微镜、粒径分析、凝胶电泳和转染实验发现,微胶囊是具有中空结构的球形胶囊,分散性良好,能够阻滞DNA并可以实现基因转染。
Microcapsules capable of loading DNA were prepared using silica (SiO 2) nanospheres as a template. Firstly, amino-adsorbed DNA was introduced into the surface of SiO 2 by using a silane coupling agent. The amino group enhanced the electrostatic attraction between SiO 2 and DNA. Subsequently, polyethyleneimine (PEA) and polyacrylic acid (PAA) were alternately adsorbed layer by layer ) To form self-assembly layer by layer to form a multi-layered polymer-loaded DNA microsphere with the template of SiO 2 nano-sphere as a template. The potential of the surface of the microsphere after layer-by-layer adsorption was tested by Zeta potential to determine the assembly proceeding. After the dissolution of SiO 2, can be obtained by carrying the DNA multi-layer polymer coated microcapsules. Finally, through transmission electron microscopy, particle size analysis, gel electrophoresis and transfection experiments found that the microcapsules are spherical capsules with hollow structure, good dispersion, can block DNA and gene transfection.