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目的:研究血虚肝郁证大鼠海马谷氨酸(Glu)及不同类型受体NR1、NR2A、NR2B、GluR1、mGluR5 mRNA的表达及芍药苷的干预作用。方法:正常组、模型组、20mg/kg芍药苷组、40mg/kg芍药苷组,造模第1天开始给药,正常组给等量超纯水,其余各组灌胃予以相应浓度药物,共造模21d。治疗结束后,取脑组织,剥离海马,采用邻苯二甲醛(OPA)柱前衍生HPLC荧光检测法测定Glu含量,采用实时荧光定量聚合酶链反应法(RTqPCR)检测NR1、NR2A、NR2B、GluR1、mGluR5等5种受体亚单位mRNA的表达。结果:血虚肝郁证候模型大鼠海马组织中Glu含量显著升高(P<0.05),且不同Glu受体亚基表达不同,其中NR2A和GluR1 mRNA的表达显著升高(P<0.01),NR1、NR2B mRNA表达有升高的趋势,而mGluR5表达无明显变化,给予芍药苷可使海马组织中Glu含量显著降低(P<0.05),可下调NR2A、GluR1、mGluR5 mRNA表达(P<0.01,P<0.05)。结论:血虚肝郁证模型大鼠脑内存在Glu能神经调节异常,白芍的养血柔肝作用,能够通过调节NO/cGMP信号通路上个的多个靶点发挥效果。
Objective: To study the expression of glutamate (Glu) and NR1, NR2A, NR2B, GluR1 and mGluR5 in hippocampus of rats with deficiency of blood and liver and the intervention of paeoniflorin. Methods: The normal group, model group, 20mg / kg paeoniflorin group and 40mg / kg paeoniflorin group were administered on the first day of modeling. The normal group was given the same amount of ultrapure water, A total of modeling 21d. At the end of treatment, the brain tissue was removed and the hippocampus was dissected. Glu content was determined by OPA column pre-column derivatization HPLC. NR1, NR2A, NR2B and GluR1 were detected by real-time fluorescence quantitative polymerase chain reaction (RTqPCR) , MGluR5 and other five kinds of receptor subunit mRNA expression. Results: The content of Glu in hippocampus of rats with blood deficiency and liver-qi stagnation syndrome was significantly increased (P <0.05), and the expression of Glu receptor subunits was different. The expressions of NR2A and GluR1 mRNA were significantly increased (P <0.01) (P <0.01). The expression of NR2A, GluR1 and mGluR5 mRNA was down-regulated in paeoniflorin group (P <0.01) , P <0.05). Conclusion: There is abnormal neuromodulation of Glu neurons in the brain of rats with deficiency of blood and liver syndrome, and the effect of nourishing blood and promoting liver of Radix Paeoniae Alba is able to exert effects by regulating multiple target sites of NO / cGMP signaling pathway.