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Sepsis can cause a series of damages to various organs of the body, so it has always been regarded as a hot research topic in veterinary clinic. Aiming at the present situation of high morbidity and mortality of canine sepsis, in order to further explore the pathogenesis of this disease, it need to establish a stable and repeatable canine sepsis model that is in line with the clinical characteristics of the disease. The study selected 12 local dogs and randomly divided into three groups: rapid bolus injection group (Group A), continuous infusion group within 30 min (Group B) and continuous infusion group (Group C). Then, the lipopolysaccharides (LPS) with 2 mg ? kg-1 were injected through the brachial vein in different modes of administration, thus the model was fully established. During the modeling period, body temperature (T), respiratory rate (RR), heart rate (HR) and mean arterial pressure (MAP) were monitored at 0, 10, 20, 30, 40, 50 min and 1, 2, 3, 4, 5, 6, 7, 8, 9, 12 and 24 h. Blood was collected from the canine brachial vein at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 12 and 24 h, respectively, for the detection of white blood cells (WBC). The test showed that the values of T, RR, HR, MAP, WBC of the dogs in group A all changed but did not exceed the normal range, and the clinical symptoms were not significant. There were no significant changes in the values of RR, HR, T, MAP and WBC of the dogs in Group B, and the clinical symptoms were not significant. The value of T of the dogs in Group C were significantly increased at 40 min (p<0.05), which reached the fever standard and lasted for 7 h; the value of RR increased significantly at 20 min (p<0.05), and a downward trend could be observed at 12 h, then it reted to normal at 24 h; the value of HR increased signi-ficantly at 50 min (p<0.05) and recovered at 8 h; the value of HR decreased significantly at 20 min (p<0.05), which remained at 12 h (p<0.05), and reted back to normal at 24 h; the value of WBC decreased significantly from 1 h to 4 h (p<0.05), which was lower than the normal value, and increased significantly at 24 h (p<0.01); all of the four dogs in this group had clinical symptoms such as vomiting, diarrhea and depression. Based on the above results, the changes of indexes and clinical symptoms in Groups A and B did not meet the standards of sepsis. After a long-term continuous intravenous infusion of LPS, the experimental dogs in Group C showed varying degrees of clinical symptoms, such as vomiting, diarrhea and depression one after another. The indexes and clinical symptoms reached the sepsis standard about 3 h after infusion. In brief, this model not only had good stability and good regularity of repeatability, but also lasted for a long time and could be suitable for other subsequent studies.