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研究媒介昆虫如何抗感染有助于制定更为成功的防制策略。本文报道了埃及伊蚊对彭亨丝虫感染免疫反应的实验结果。1.免疫血淋巴对微丝蚴的体外杀伤作用从经微丝蚴免疫的不易感的埃及伊蚊中取血淋巴,分别与抗已知昆虫免疫蛋白(Sar-cotoxin IA,ILA和Sarcolectin)的特异抗体一起孵育过夜。抗体来自提纯的麻蝇蛋白,抗体稀释为1:30,血淋巴在含HEPES的TC199缓冲液中稀释为1:3。将3μl抗体/抗原
Studying how vector insects fight infections helps to develop more successful strategies for control. This paper reports the experimental results of Aedes aegypti on immune response to Paenibacillus. In vitro cytotoxicity of the immune hemolymph against microfilariae The hemolymph was harvested from the non-susceptible Aedes aegypti as immunized with microfilariae, and incubated with anti-HIV antibodies against the known insect immune proteins (Sar-cotoxin IA, ILA and Sarcolectin) Specific antibodies were incubated overnight. Antibodies were from purified flies protein diluted 1:30 in antibody and hemolymph diluted 1: 3 in HEPES-containing TC199 buffer. 3 μl of antibody / antigen