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采用冠状动脉旁路术治疗缺血性心脏病,约有80～90％病人术后心绞痛获得缓解并延长寿命。大隐静脉移植失败的原因主要是移植血管本身硬化和血栓形成所致,这除了吻合口技术问题外,尚有多种涉及生化的因素,有些与血管段本身性质有关,故作者认为有分析移植血管段代谢情况的必要。作者从19例冠状动脉旁路手术病人身上取下新鲜大隐静脉,将其标本快速冰冻至-45℃,或立即洗去所有结缔组织,吸干、秤量、切碎后用PT-20磷酸盐缓冲
Coronary artery bypass surgery for ischemic heart disease, about 80 ~ 90% of patients after angina pectoris and prolong life expectancy. The main reason for the failure of transplantation of saphenous vein is mainly due to the hardening of blood vessels and the thrombosis. In addition to the technical problems of anastomosis, there are many factors involved in biochemical and biochemical factors, some of which are related to the nature of vascular segments. Therefore, Necrosis of blood vessels is necessary. The authors removed fresh saphenous vein from 19 patients undergoing coronary artery bypass surgery, rapidly frozen their specimens to -45 ° C, or immediately washed away all connective tissue, blotted, weighed, shredded and treated with PT-20 phosphate buffer