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采用聚丙烯酸胺凝胶电泳方法，从栗疫病菌Cryphonectriaparasitica的18种酶体系中筛选到9种分辨率高的同工酶。对同工酶的检测结果表明，在中国的北京、辽宁、江西、福建及意大利、美国、日本共7个群体的115个栗疫病菌菌株中，多酚氧化酶（PPO）、过氧化物酶（POX）、细胞色素氧化酶（CCO）、乙醇脱氢酶（ADH）、超氧化物歧化酶（SOD）为单态性；α-酯酶（α－EST）、β-酯酶（β-EST）、苹果酸脱氢酶（MDH）、苹果酸酶（ME）为多态性，分别具有10、7、3、3种电泳表现型。通过9种同工酶的凝胶电泳分析，对栗疫病菌的群体结构进行了比较。中国地区4个群体间的遗传相似性较大，与美国、意大利、日本群体间的相似性较小；美国、意大利群体间的遗传相似性较大，且它们与日本群体间的相似性大于与中国各群体间的相似性，这一结果表明美国的栗疫苗可能是从日本传人的。病菌群体的遗传变异率为of60，其中83．1％由群体内的变异引起，16．9％由群体间的差异引起。群体间的基因流动值为246。
Polyacrylamide gel electrophoresis was used to screen 9 isozymes with high resolution from 18 enzyme systems of Cryphonectria parasitica. The results of isozymes showed that in 115 pathogenic strains of CBZ from 7 populations in Beijing, Liaoning, Jiangxi, Fujian, Italy, the United States and Japan in China, polyphenol oxidase (PPO), peroxidase (POX), cytochrome oxidase (CCO), alcohol dehydrogenase (ADH) and superoxide dismutase (SOD) EST), malate dehydrogenase (MDH) and malate enzyme (ME) were polymorphic, with 10, 7, 3 and 3 electrophoretic phenotypes respectively. The population structure of Cryphonecarcoides was compared through gel electrophoresis analysis of nine isozymes. The genetic similarity among the four populations in China is relatively large, but less similar to those in the United States, Italy and Japan. The genetic similarities among the populations of the United States and Italy are greater, and their similarities with Japanese groups are greater than The similarities among Chinese groups suggest that the chestnut vaccine in the United States may be transmitted from Japan. The genetic variation rate of the pathogen population was of60, of which 83.1% was caused by variation within the population and 16.9% due to the difference between the populations. Gene flow between populations was 246.