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本文报告在国内首次建成了胚肺细胞-伯氏疟原虫红外期体外培养系统.用胰酶消化法自人工流产胎儿分离出胚肺细胞后,建立Elu 8801细胞株。斯氏按蚊叮咬伯氏疟原虫ANKA株感染的昆明小鼠18～21d后,在无菌条件下解剖出唾腺,制备子孢子悬液,接种于单层培养的胚肺细胞。培养48h后,可见红外期裂殖体。72h后,部分裂殖体内已形成成熟的裂殖子,将培养上清经腹腔接种健康小鼠,可使之感染疟疾并可经血传感染其它小鼠。用第2代感染小鼠血饲喂按蚊,蚊体内可产生子孢子。表明本室建立的人胚肺细胞林对P.b.ANKA株易感染,并能支持其红外期发育成熟,产生有感染力的红外期裂殖子。
In this paper, we report the first in vitro incubation system of embryo lung-Plasmodium berghei in vitro.Elu 8801 cells were established by isolating embryonic lung cells from human abortion fetuses using trypsin digestion method. Anopheles stephensi bites P. berghei ANKA strain infected Kunming mice 18 to 21 days later, the salivary glands are dissected under aseptic conditions, and sporocysts are prepared and inoculated into monolayer culture embryonic lung cells. 48h after culture, we can see the inflorescence schizonts. After 72h, mature merozoites were formed in some schizonts. The cultured supernatants were intraperitoneally inoculated into healthy mice to infect malaria and blood transfection to infect other mice. With the second generation of infected mice blood feeding Anopheles mosquitoes can produce sporozoites. This indicates that the human embryo lung cell line established in our laboratory is susceptible to P.b.ANKA strain and can support its maturity in infra-red period to produce infectious merozoites in infra-red stage.