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对虾WSSV病是亚洲对虾养殖业中的一个棘手问题。本研究采用Kimura引物 ,用PCR技术对不同生长期的中国对虾 (Penaeuschinensis)进行了WSSV的检测 ,同时也检测了对虾发病时养殖池中多见的野生厚蟹 (Helicesp .)和矛尾刺虎鱼 (Acanthogobiushasta)。检测结果表明 :分别在检测的 5尾亲虾中的 1尾 ,6尾仔虾中的 1尾 ,5尾稚虾中的 3尾及所检测的 5尾病虾和 2只厚蟹中获得到 982bp的PCR扩增产物 ,说明为WSSV感染阳性。在检测的 2尾矛尾刺虎鱼中均未获得PCR扩增产物 ,说明为WSSV感染阴性。在亲虾、虾苗以及虾池内的野生厚蟹中检测到WSSV感染的阳性结果表明 :WSSV感染的亲虾有可能是病毒的储主 ,WSSV感染的野生厚蟹有可能是病毒中间宿主或病毒的携带者 ,它们在对虾WSSV病的感染、传播中起了重要的作用
WSSV disease in shrimp is a thorny issue in the shrimp aquaculture industry in Asia. In this study, Kimura primers were used to detect the WSSV of Penaeus chinensis in different growth stages by PCR, and also to detect the presence of more common wild crab (Helicesp.) And Spearfish Acanthogobiushasta. The results showed that one of the 5 tested broodstock, one of the six tail larvae, three of the five tail juveniles, and five of the five dead shrimp and two of the tested crabs were detected 982bp PCR amplification product, indicating positive for WSSV infection. No PCR amplification products were detected in the two tested goby tailed gobies, indicating negative for WSSV infection. The positive results of WSSV infection in broodstock, shrimp seedlings and wild thick crabs in shrimp ponds indicated that WSSV-infected broodstock may be the host of virus and WSSV-infected wild thick crab may be the virus intermediate host or virus Of carriers, which play an important role in the infection and transmission of WSSV in shrimp