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通过土施和喷施不同浓度亚硒酸钠的盆栽及大田实验,研究不同浓度的外源硒肥对茶树叶中硒代氨基酸形态及含量的影响;采用离子色谱-积分脉冲安培检测法(HPAEC-IPAD)对茶树叶中硒代氨基酸进行定性和定量分析。结果显示:3种硒代氨基酸在其线性范围内的相关系数R2在0.9994～0.9999之间,相对标准偏差RSD(%)分别为2.47、3.17和3.77,具有很好的相关性;茶树叶中硒代甲基半胱氨酸(SeMeCys)是硒代氨基酸存在的主要形式,随着喷施和土施亚硒酸钠浓度的增加,其含量呈现先增加后降低的趋势,茶树叶中总的硒代氨基酸的含量变化与此变化相符;茶树叶中总的硒代氨基酸在喷施Se180μg ml-1、12天左右时采摘最为理想,所含硒代氨基酸总量为336.1μg g-1,但喷施浓度不宜高于Se 180μg ml-1,否则茶树叶面上会出现褐色的斑点及焦化,影响其外观和品质;土施亚硒酸钠时以Se 0.25～0.5 mg kg-1,施后6个月采摘较佳,茶树叶中总的硒代氨基酸的含量为293.9～471.7μg g-1,大田试验结果与盆栽试验结果相符。
Effects of different concentrations of exogenous selenium fertilizer on the morphology and content of seleno-amino acids in tea leaves were studied by pot experiment and field experiment with different concentrations of sodium selenite and soil spraying. -IPAD) qualitative and quantitative analysis of seleno amino acids in tea leaves. The results showed that the correlation coefficients (R2) of three kinds of selenoamino acids in the linear range were between 0.9994 and 0.9999, and the relative standard deviations (RSDs) were 2.47, 3.17 and 3.77, respectively. Methyl cysteine (SeMeCys) is the main form of seleno-amino acids present, with spraying and soil sodium selenite concentration increased, the content of the first increased and then decreased, the total selenium in tea leaves The amino acid content of the amino acids in the leaves was consistent with this change. The total selenoamino acids in the leaves of tea plants were optimally picked at Se180μg ml-1 for about 12 days, and the total amount of seleno-amino acids contained was 336.1μg g-1, Shi concentration should not be higher than Se 180μg ml-1, or tea leaves will appear brown spots and coking, affecting its appearance and quality; soil selenite Se 0.25 ~ 0.5 mg kg-1, after 6 Picking the best month, the total content of seleno-amino acids in tea leaves was 293.9 ~ 471.7μg g-1, field test results and pot test results.