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目的 探讨重症肌无力 ( MG)患者乙酰胆碱受体 ( ACh R)特异性 T细胞免疫应答及其与乙酰胆碱受体抗体 ( ACh RAb)间的关系。方法 应用体外细胞培养和 ELISA方法检测 MG患者外周血单个核细胞 ( PBMNC)经 ACh R刺激后产生 IFN-γ和 IL-4的水平。结果 MG患者 IFN-γ和 IL-4的诱生水平均比对照组明显升高( P<0 .0 1 ) ;ACh RAb阳性组 MG患者 IFN-γ和 IL-4的诱生水平明显高于 ACh RAb阴性组 ( P<0 .0 1和 P<0 .0 5 )。结论 MG患者无论为 ACh RAb阳性或阴性 ,均存在 ACh R特异性 T细胞激活 ,IFN-γ和 IL-4均与自身抗体的产生有关。
Objective To investigate the ACh R-specific T cell immune response and its relationship with acetylcholine receptor antibody (ACh RAb) in patients with myasthenia gravis (MG). Methods The levels of IFN-γ and IL-4 in peripheral blood mononuclear cells (PBMNC) stimulated by ACh R in MG patients were detected by in vitro cell culture and ELISA. Results The induced levels of IFN-γ and IL-4 in MG patients were significantly higher than those in control subjects (P <0.01). The levels of IFN-γ and IL-4 in MG patients with ACh RAb were significantly higher than those in MG patients ACh RAb negative group (P <0.01 and P <0.05). Conclusion ACh R-specific T cell activation exists in both MG patients with ACh RAb positive and negative. Both IFN-γ and IL-4 are associated with the production of autoantibodies.